This is the first report implementing combined density gradient centrifugation/Zeta (DGC/Zeta) sperm selection procedure for a couple with eleven previous intra cytoplasmic sperm injection/ in vitro fertilization (ICSI/IVF) failure cycles. Semen analysis was carried out according to World Health Organization criteria. Protamine deficiency, DNA fragmentation and morphology were assessed by chrom...

In this paper, the development of a computer controlled piezo manipulation system for biomedical applications such as intra-cytoplasmic sperm injection (ICSI) is presented. The hardware setup and the control strategies will be described in full details to illustrate the advantages of this approach as compared to other manual-based injection methods.

Background In vitro fertilization (IVF) and intra cytoplasmic sperm injection (ICSI) are recognized as established and increasingly successful forms of treatment for infertility, yet significant numbers of couples discontinue treatment without achieving a live birth. This study aims to identify major factors that influence the decision to discontinue IVF/ICSI treatments. MaterialsAndMethods We ...

During the last decades, many studies have shown the possible influence of sperm DNA fragmentation on assisted reproductive technique outcomes. However, little is known about the impact of sperm DNA fragmentation on the clinical outcome of frozen-thawed embryo transfer (FET) from cycles of conventional in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI). In the present stu...

The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried ...

Background The aim of this study was to compare prenatal outcome of intra cytoplasmic sperm injection (ICSI) pregnancies with pregnancies obtained through in vitro fertilization (IVF). MaterialsAndMethods Retrospectively 532 pregnancies of ART cycles were assessed during 1999-2000. The main outcome measured including abortions, ectopic pregnancies, prematurity, low birth weight, cesarean sectio...

BACKGROUND Gonadotropin-releasing hormone agonists or antagonists are used in assisted reproductive technique cycles as premature luteinizing hormone secretion inhibition. Studies have been reported different and contradictory results on the serum progesterone effect on intra-cytoplasmic sperm injection. OBJECTIVE The purpose of this study was to evaluate the effect of serum progesterone leve...

assessment of embryo quality in order to choose the embryos that most likely result in pregnancy is the critical goal in assisted reproductive technologies (art). the current trend in human in vitro fertilization/embryo transfer (ivf/et) protocols is to decrease the rate of multiple pregnancies after multiple embryo transfer with maintaining the pregnancy rate at admissible levels (according to...