abstract- desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. a pcr-based method was developed for the identification of 3 major desert truffles of iran: terfezia claveryi, tirmania pinoyi and tirmania nivea based on internal transcribed spacers of rdna. two specific pcr primers were designed for t. claveryi, 4 for t. pinoyi, and 2 for ...

Pleosporaceae is an important Dothideomycetes family. To elucidate relationships among some selected anamorphic pleosporalean taxa, their Internal Transcribed Spacer (ITS) and RNA polymerase second largest subunit (RPB2) were sequenced and compared. Phylogenetic analyses of both ITS and RPB2 regions were almost similar and generally congruent with previously described phylogenies and morphology...

Social & Scientific Systems, Inc., Durham, NC 27713, USA Integrated Laboratory Systems, Inc., Research Triangle Park, NC 27709, USA National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA Procter & Gamble NV, Stro...

DNA barcode is a very short, standardized DNA sequence in a well-known gene. It provides a way to identify the species to which a plant, animal or fungus it belongs. One of the most popular sequences identification is achieved by comparing DNA sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal cistron. DNA barcoding is a new concept of identifying unknown organisms b...

DNA sequence divergence at internal transcribed spacer regions (ITS-1 and ITS-2) was compared with divergence at mitochondrial cox1 or nad4 loci in pairs of congeneric nematode species. Mitochondrial sequences accumulate substitutions much more quickly than internal transcribed spacer, the difference being most striking in the most closely related species pairs. Thus, mitochondrial DNA may be t...

Ficus species (Moraceae) is a globally distributed and considered one of the largest most diverse genera woody plants with growing genetic, environmental, economic, nutritional, medicinal importance. This study was conducted to detect genetic evolutionary relationships among eight genus (Ficus microcarpa var nitida, F. retusa, benjamina Vivian, Starlight, cyathistipula, elastica Decora binnendi...