Molecular dynamics simulations have been used to calculate the potentials of mean force for separating short cellooligomers in aqueous solution as a means of estimating the contributions of hydrophobic stacking and hydrogen bonding to the insolubility of crystalline cellulose. A series of four potential of mean force (pmf) calculations for glucose, cellobiose, cellotriose, and cellotetraose in ...

Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method to explore the detergent resistance of an important G-protein coupled recepto...

The quantum mechanical measurement problem is the difficulty of dealing with the indefiniteness of the pointer observable at the conclusion of a measurement process governed by unitary quantum dynamics. There has been hope to solve this problem by eliminating idealizations from the characterization of measurement. We state and prove two ‘insolubility theorems’ that disappoint this hope. In both...

I present a very general and simple argument — based on the no-signalling theorem — showing that within the framework of the unitary Schrödinger equation it is impossible to reproduce the phenomenological description of quantum mechanical measurements (in particular the collapse of the state of the measured system) by assuming a suitable mixed initial state of the apparatus. The thrust of the a...

phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...

Glycosylphosphatidylinositol (GPI)-anchored proteins are poorly solublized in non-ionic detergents such as Triton X-100 and Nonidet P40, but are easily solublized by detergents with high critical micelle concentrations such as octylglucoside. This solubility profile has been suggested to be due to the localization of GPI-anchored proteins to lipid microdomains rich in cholesterol and sphingolip...

The phenomenon of detergent insolubility of bovine hippocampal membranes in Triton X-100 was monitored by estimating the presence of phospholipids in the insoluble pellet. This represents a convenient and unambiguous assay and reports the dependence of the extent of phospholipid solubilization on detergent concentration. The advantage of this approach is its ability to accurately determine the ...

The rotavirus NSP5 protein directs the formation of viroplasm-like structures (VLS) and is required for viroplasm formation within infected cells. In this report, we have defined signals within the C-terminal 21 amino acids of NSP5 that are required for VLS formation and that direct the insolubility and hyperphosphorylation of NSP5. Deleting C-terminal residues of NSP5 dramatically increased th...