Figure 3. Comparison of BSA crosslinking efficiency by SDS-PAGE. Different crosslinkers were incubated with BSA at molar excess of crosslinker to protein (e.g. 20, 100 or 500-fold). Crosslinking efficiency is shown by decreased mobility by SDS-PAGE and varied by crosslinker type, solubility and concentration. Figure 1. Structures of non-cleavable and MS-cleavable crosslinkers used for protein –...

This paper presents a pH-sensitive bifunctional crosslinker that enables facile conjugation of small molecule therapeutics to macromolecular carriers for use in drug delivery systems. This N-ethoxybenzylimidazole (NEBI) bifunctional crosslinker was designed to exploit mildly acidic, subcellular environments to trigger the release of therapeutics upon internalization in cells. We demonstrate tha...

Using a new procedure developed in our laboratory for the preparation of thick polymethacrylate films bonded in fused silica capillaries, crosslinked polymeric stationary phases were synthesized in situ in 25-mm-i.d. capillaries. The effect of monomer and crosslinker concentrations on the resulting polymer film Ž . was studied by open tubular capillary electrokinetic chromatography OTCEC Ž . us...

BACKGROUND Large conformational and functional changes of azobenzene-modified biomolecules require longer azobenzene derivatives that undergo large end-to-end distance changes upon photoisomerization. In addition, isomerization that occurs with visible rather than UV irradiation is preferred for biological applications. RESULTS We report the synthesis and characterization of a new crosslinker...

Nanoformulation consisting of Streptomycin loaded chitosan-alginate nanoparticles were prepared using ionotropic-pregelation method and optimization was done in terms of polymer concentration, crosslinker concentration and stirring time. The optimal parameters were found to be Chitosan 0.75mg/ml, calcium chloride 1% (w/v) and stirring time 90 min. Polymer (chitosan and sodium-alginate) and cros...

Information on the preparation and properties of starch/gelatin blend microparticles with and without crosslinking for drug delivery is presented. The blend microparticles were prepared by the water-in-oil emulsion solvent diffusion method. Glutaraldehyde and methylene blue were used as the crosslinker and the water-soluble drug model, respectively. The blend microparticles were characterized b...

A resin monomer-soluble polyrotaxane (PRX) crosslinker with cleavable end groups was synthesized to develop degradable photosetting composite resins. The PRX containing 50 α-cyclodextrins (α-CDs) with disulfide end groups was initially modified with n-butylamine to obtain a resin monomer-soluble PRX. The PRX containing 13 n-butyl groups per α-CD molecule was completely soluble in conventional r...

Crosslinking mass spectrometry has become a core technology in structural biology and is expanding its reach towards systems biology. Its appeal lies rapid workflow, high sensitivity the ability to provide data on proteins complex systems, even whole cells. The depends heavily crosslinking reagents. anatomy of crosslinkers can be modular, sometimes comprising combinations functional groups. The...

The effects of the monomer ratio, surfactant, and crosslinker contents on the particle size and phase-transition behavior of the copolymer poly(N-isopropylacrylamide-co-methacrylic acid) (PNIPAAm–MAA) were investigated with Fourier transform infrared, differential scanning calorimetry, and dynamic laser scattering techniques. In addition to the thermoresponsive property of poly(N-isopropylacryl...