The aerobic oxidation of indole-3-acetic acid catalyzed by horseradish peroxidase produces 1268 nm emission characteristic of singlet oxygen. Lactoperoxidase also oxidizes indole-3-acetic acid to produce singlet oxygen, but in contrast to horseradish peroxidase, this enzyme system requires hydrogen peroxide. In both of these systems, the intensity of the 1268 nm emission is small due to quenchi...

Incubation of mature sweet corn kernels of Zea mays in dilute solutions of (14)C-labeled indole-3-acetic acid leads to the formation of (14)C-labeled esters of myo-inositol, glucose, and glucans. Utilizing this knowledge it was found that an enzyme preparation from immature sweet corn kernels of Zea mays catalyzed the CoA- and ATP-dependent esterification of indole-3-acetic acid to myo-inositol...

We characterized the biosynthesis of indole-3-acetic acid by the mycoherbicide Colletotrichum gloeosporioides f. sp. aeschynomene. Auxin production was tryptophan dependent. Compounds from the indole-3-acetamide and indole-3-pyruvic acid pathways were detected in culture filtrates. Feeding experiments and in vitro assay confirmed the presence of both pathways. Indole-3-acetamide was the major p...

Homogenates of pea (Pisum sativum L., var. Alaska) seedlings exposed to (14)C-indole-3-acetic acid or (14)C-3-methyleneoxindole, an oxidation product of indole-3-acetic acid, were extracted with phenol. In both cases 90% of the bound radioactivity was found associated with the protein fraction and 10% with the water-soluble, ethanol-insoluble fraction. The binding of radioactivity from (14)C-in...

A flexible analytical system which allows for the continuous potentiometric monitoring of the disappearance of an electrochemical species, ferrocyanide, by the peroxidase enzyme is described. The ability of peroxidase to mediate the oxidation of indole-3-acetic acid is followed by observing the competition of indole-3-acetic acid with ferrocyanide for the peroxidase enzyme. This is accomplished...

A direct radioimmunoassay of the methyl ester of urinary and serum 5-hydroxy-3-indole acetic acid is described. The antiserum, raised in a rabbit against a conjugate of bovine serum albumin with 5-hydroxytryptamine hemisuccinamide, contained two antigenic fractions, one binding N-acyl 5-hydroxytryptamine, and the other binding methyl ester of 5-hydroxy-3-indole acetic acid, and N-acyl 5-hydroxy...

Indole-a-acetic acid is well known as an inducer as well as an inhibitor of several growth and differentiative processes in plants, lower metazoa, and microorganisms. The principle of unity in biology suggests that these diverse effects of indole-3-acetic acid may all be consequences of interference with a common reaction or series of reactions; therefore, the study of the action of indolea-ace...

indole acetic acid is an important hormone that improved the growth of different plant organs such as the root under normal and stress conditions. as various studies have shown, most of rhizobacteria produce indole acetic acid and thereby contribute to the growth of plants. in this study, different rhizobacteria were isolated from different regions of iran for the assessment of their ability to...

The recently reported growth-promoting ability of 3-methyl-eneoxindole was examined in order to test the hypothesis that indole-3-acetic acid acts as a growth promoter only after oxidative conversion to 3-methyleneoxindole. Methyleneoxindole was synthesized from indole-3-acetic acid and N-bromosuccinimide, and its identity was confirmed by ultraviolet absorption, infrared absorption, mass spect...