COMPARISON OF ENZYME IMMUNOASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS. The indirect enzyme immunoassay for measurement of bovine antibody to Brucella abortus was tested on 15,716 Canadian sera to assess the specificity. These sera were also tested by the buffered plate antigen test. Two ELISA formats were used for assessment of data: the targeting procedure using a positive control serum al...

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for a screening test to detect antibodies against classical swine fever virus (CSFV). Viral glycoproteins, which were purified from swine kidney cells infected with CSFV ALD/A76 strain by the immunoaffinity purification using monoclonal antibody against E2 protein, were adsorbed on a microtiter plate as the antigen for the anti...

Brucellosis is one of the most important reemerging zoonoses in many countries. Brucellosis is caused by Gram-negative coccobacillus belonging to genus Brucella. Human brucellosis often makes the diagnosis difficult. The symptoms and clinical signs most commonly reported are fever, fatigue, malaise, chills, sweats headaches, myalgia, arthralgia, and weight loss. Some cases have been presented w...

APPLICATION OF ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN RIO GRANDE DO SUL, BRAZIL. The results of an indirect ELISA (I-ELISA) and a competitive ELISA (C-ELISA) for detection of antibody to Brucella abortus in cattle were compared with those of conventional serological tests. The sensitivity of I-ELISA using 230 sera from infected animals was 98.6% and the sp...

Background: Leptospirosis is a zoonotic bacterial disease causing abortion, stillbirths and loss of milk production in livestock resulting huge economic losses. cattle has been under-reported under-diagnosed India due to non-specific symptoms, complex laboratory tests, fastidious nature bacterium lack proper diagnostic facilities. Scanty data pertaining epidemiological status leptospirosis avai...

Three murine monoclonal IgG1 kappa-antibodies, MAH-1, MAH-2, and MAH-3, were raised against factor H purified from human plasma. In cross-inhibition studies MAH-3 did not compete with MAH-1 and MAH-2, and vice versa, for the binding to H, whereas MAH-1 and MAH-2 inhibited each other. MAH-1 and MAH-2 inhibited the binding of H to C3b attached to an ELISA plate as well as to C3b bound to sheep er...

Brucellosis is an important zoonotic infectious disease of humans and livestock with worldwide distribution and is caused by bacteria of the genus Brucella. The diagnosis of brucellosis always requires laboratory confirmation by either isolation of pathogens or detection of specific antibodies. The conventional serological tests available for the diagnosis of brucellosis are less specific and s...

AIM Determine the optimal cut-off and the use of Fluorescence Polarization Assay (FPA) to improve the detection of brucellosis in individual goats in Argentina. METHODS Sera from 96 goats from a flock with abortion due to B. melitensis biovar 1 were used to assess the efficacy of the FPA to detect brucellosis in goats. FPA results were compared with those of the Buffered Antigen Plate Aggluti...

Aim: To study the seroprevalence of caprine bruellosis in Karnataka and compare the relative sensitivity and specificity among the different serological tests used. Materials and Methods: A total of 252 serum samples were collected from the goats of Karnataka and subjected to 5 different serological tests, i.e., Rose Bengal Plate Test (RBPT), Standard Tube Agglutination Test (STAT), 2-mercaptoe...