The distribution of binding sites for rabbit anti-species antiserum, Concanavalin A (Con A) and peanut agglutinin (PNA) on dissociated blastomeres from 2- to 16-cell mouse embryos has been investigated using direct and indirect fluorescence techniques. With each ligand, paraformaldehyde-fixed blastomeres from 2- to 8-cell precompact embryos were uniformly surface labelled; the majority (77%) o...

This paper describes a series of French semantic role labelling experiments which show that a small set of manually annotated training data is superior to a much larger set containing semantic role labels which have been projected from a source language via word alignment. Using universal part-of-speech tags and dependencies makes little difference over the original fine-grained tagset and depe...

An oligonucleotide probe was selected from the 16S rRNA gene of Clavibacter michiganensis subsp. sepedonicus for specific in situ hybridization. The rhodamine-labelled oligonucleotide probe was used in conjunction with an indirect immunofluorescence procedure based on a specific monoclonal antibody detected with a fluorescein-labelled conjugate. Simultaneous labelling of bacterial cells with th...

Pig liver oligosaccharyltransferase (OST) is inactivated irreversibly by a hexapeptide in which threonine has been substituted by epoxyethylglycine in the Asn-Xaa-Thr glycosylation triplet. Incubation of the enzyme in the presence of Dol-PP-linked [14C]oligosaccharides and the N-3,5-dinitrobenzoylated epoxy derivative leads to the double-labelling of two subunits (48 and 66 kDa) of the oligomer...

The human erythrocyte membrane skeleton consists of a network of short actin filaments cross-linked into a hexagonal network by long, flexible spectrin molecules. The lengths of the short actin filaments (33 +/- 5 nm) at the central junctions are proposed to be stabilized and limited by association with tropomyosin and the tropomyosin-binding protein, tropomodulin. Here, we use immunogold label...

Much indirect evidence supports the assumption that tritiated thymidine does not label cells which enter the deoxyribonucleic acid synthesis phase (S) more than 1 h after injection. Direct evidence confirming this assumption was obtained by counting labelled epithelial nuclei in mice killed i, 4 or 6 h after a single intraperitoneal injection of [H]thymidine; colchicine was used to prevent the ...

We describe a robust approach for linking already existing lexical/semantic hierarchies. We use a constraint satisfaction algorithm (relaxation labelling) to select –among a set of candidates– the node in a target taxonomy that bests matches each node in a source taxonomy. In this paper we present the complete mapping of the nominal, verbal, adjectival and adverbial parts of WordNet 1.5 onto Wo...