conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an i...

alkylhydroperoxide reductase (ahpc, the 26 kda antigen) is one of the abundant antioxidant enzymes in helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect h. pylori infection in clinical specimens. this study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. five established hybridoma cell lines secreti...

background: toxoplasma gondii is an obligatory intracellular protozoan parasite on which studies are pending regarding production of vaccine. to date, the production of human vaccine has not been successful where approximately one third of the world’s population is thought to be infected with t. gondii. objectives: the present study was designed to compare the electrophoretic patterns of t. gon...

 This study was aimed to detect anti-Toxoplasma gondii antibodies in cats infected with Rh strain of T. gondii by using an immunoblotting method. For this, cats were experimentally infected using tachyzoites harvested from Vero cell cultures. Tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to PVDF membranes. An immunoblotting was performed using these mem...

To differentiate infectious endocarditis (IE) from other Bartonella infections and to identify infecting Bartonella bacteria at the species level on a serological basis, we used Western immunoblotting to test sera from 51 patients with Bartonella IE (of which 27 had previously benefited from species identification by molecular techniques), 11 patients with chronic Bartonella quintana bacteremia...