background: considering the importance of ticks as a main group transmitting pathogen organisms, this study de­signed to recognize immunogenic proteins in different tissues of rhipicephalus ( boophilus ) annulatus tick and to find out if there are common proteins in these tissues. methods: seven cattle were experimentally infested with about 10000 r. annulatus larvae and their humoral im­mune r...

Purpose: To investigate the expression of reticulocalbin-2 (RCN2) in human oral squamous cell carcinoma (OSCC) tissues and lines its effects on proliferation migration OSCC cells.Methods: Immunohistochemical (IHC) assays were performed to evaluate RCN2 30 histologically-confirmed patient adjacent tissues. Immunoblot examine an mucosal keratinocyte line lines, while assessed by CCK-8 colony form...

Extracellular signal-regulated kinase (ERK) has been implicated in mammalian testicular and epididymal development. This study aimed to investigate ERK expression the immature mature testes epididymides of bulls. We evaluated using immunoblot analysis immunohistochemistry. Immunoblot revealed that bull had higher phosphorylated (pERK) than epididymides. pERK immunoreactivity was testes. loc...

Two simple Bartonella bacilliformis immunoblot preparation methods were developed. Antigen was prepared by two different methods: sonication of whole organisms or glycine extraction. Both methods were then tested for sensitivity and specificity. Well-defined control sera were utilized in the development of these diagnostic immunoblots, and possible cross-reactions were thoroughly examined. Sera...

Supplemental tests using Immunoblot are recommended to improve specificity of anti-HCV by ELISA. In Brazil immunoblot is not officially recommended. Aiming to identify EIA false-positive rate 70 positive EIA anti-HCV blood donors were submitted to 3rd generation immunoblot at Hemocentro of Mato Grosso State where polymerase chain reaction tests are not performed. There were 44 (62.9%) immunoblo...

Purpose: To determine the potential effect of Chikusetsu saponin IVA (CS-IVA) on benign prostatic hyperplasia (BPH), as well mechanism action.
 Methods: Benign epithelial cell response to treatment by CS-IVA was evaluated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation assay, and cycle analysis. Flow cytometry immunoblot assay were used assess apoptosis...

We present a factor analytic study of the Conners Rating Scales for parents and teachers in this article. A comparison is established with the original factor analytic studies (Conners, 1979a, 1979b) and the results obtained by Farré-Riba and Narbona (1997), using a Spanish sample. Five hundred and forty children, ages 4 to 17, were randomly selected in Manizales, Colombia. The shortened Spanis...