Competitive immunoassays are important diagnostic assays for the detection of small molecules such as vitamins, minerals, or some hormones.

Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...

Abstract Immunoassays are a standard diagnostic tool that assesses immunity in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection. However, immunoassays do not provide information about contaminating antigens or cross-reactions and might exhibit inaccurately high sensitivity low specificity. We aimed to gain insight into the serological immune response of SARS-CoV-2 pat...

Two commercial monoclonal immunoassays for monitoring cyclosporin A were used to measure whole-blood concentrations of the immunosuppressant cyclosporin G (CsG) in renal transplant patients. We performed a three-way comparison of these two immunoassays and HPLC. Although the two immunoassays agreed favorably, both the monoclonal fluorescence polarization immunoassay and the monoclonal RIA yield...

Immunoassay has been widely used for quantification of proteins and small molecules in medical diagnostics, proteomics, drug discovery, and various biological research.1 ELISA (Enzyme-Linked ImmunoSorbant Assay) is the most commonly used immunoassay format.2 Traditional ELISA assays used in clinical settings are laborious and expensive and often consume large quantities of reagents and patient ...

Background: The quality of testosterone assays has been amatter of debate for several years. Known limitations of testosterone immunoassays are the cross-reactivity with other steroids and a high variation in the low concentration range. We hypothesized that one of the additional limitations of testosterone immunoassays is an ineffective displacement of testosterone from its binding protein. Me...

OBJECTIVES Microparticle-enhanced nephelometric immunoassays for six human milk proteins (beta-casein, kappa-casein, alpha-lactalbumin, serum albumin, lactoferrin, and lysozyme) and conventional immunonephelometry assays for immunoglobulin A, C3, and C4 complement proteins were developed and characterized. DESIGN AND METHODS Microparticle-enhanced nephelometric immunoassays are competitive as...