نتایج جستجو برای: immucillin a

تعداد نتایج: 13431800  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2013
Mathew J Vetticatt Boris Itin Gary B Evans Vern L Schramm

Transition state analogs mimic the geometry and electronics of the transition state of enzymatic reactions. These molecules bind to the active site of the enzyme much tighter than substrate and are powerful noncovalent inhibitors. Immucillin-H (ImmH) and 4'-deaza-1'-aza-2'-deoxy-9-methylene Immucillin-H (DADMe-ImmH) are picomolar inhibitors of human purine nucleoside phosphorylase (hPNP). Altho...

2013
Christopher F Stratton Vern L Schramm

Purine nucleoside phosphorylase (PNP; EC: 2.4.2.1) is a key enzyme involved in the purine salvage pathway. A recent bioinformatic study by Yadav, P. K. et al. (Bioinformation 2012, 8(14), 664-672) reports PNP as an essential enzyme and potential drug target in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). We conducted an analysis using the methodology outlined by the...

2011
María B. Cassera Keith Z. Hazleton Emilio F. Merino Nicanor Obaldia Meng-Chiao Ho Andrew S. Murkin Richard DePinto Jemy A. Gutierrez Steven C. Almo Gary B. Evans Yarlagadda S. Babu Vern L. Schramm

Plasmodium falciparum causes most of the one million annual deaths from malaria. Drug resistance is widespread and novel agents against new targets are needed to support combination-therapy approaches promoted by the World Health Organization. Plasmodium species are purine auxotrophs. Blocking purine nucleoside phosphorylase (PNP) kills cultured parasites by purine starvation. DADMe-Immucillin-...

Journal: :Biochemistry 2007
Erika A Taylor Agnes Rinaldo-Matthis Lei Li Mahmoud Ghanem Keith Z Hazleton María B Cassera Steven C Almo Vern L Schramm

The purine salvage pathway of Anopheles gambiae, a mosquito that transmits malaria, has been identified in genome searches on the basis of sequence homology with characterized enzymes. Purine nucleoside phosphorylase (PNP) is a target for the development of therapeutic agents in humans and purine auxotrophs, including malarial parasites. The PNP from Anopheles gambiae (AgPNP) was expressed in E...

Journal: :Biochemistry 1998
R W Miles P C Tyler R H Furneaux C K Bagdassarian V L Schramm

Genetic defects in human purine nucleoside phosphorylase cause T-cell deficiency as the major phenotype. It has been proposed that efficient inhibitors of the enzyme might intervene in disorders of T-cell function. Compounds with features of the transition-state structure of purine nucleoside phosphorylase were synthesized and tested as inhibitors. The transition-state structure for purine nucl...

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