In this study, immobilization of partially purified almond (Amygdalus communis) β-galactosidase on Con A layered calcium alginate-cellulose beads was investigated. Immobilized β-galactosidase retained 72% of theinitial activity after crosslinking by glutaraldehyde. Both soluble and immobilized enzyme exhibited the samepH and temperature optima at pH 5.5 and 50ºC, respectively. Howev...

An overview of the use of immobilized enzymes in flow-injection analysis (FIA) is presented. The joint use of FIA and immobilized enzymes means that analytical procedures are easily automated, analytical costs are reduced and methods are faster. The future possibilities for this combination are discussed.

alkaline protease enzyme produced from bacillus licheniformis ptcc1331 in optimum condition(temperature 37 ?c, 150 rpm, ph=10), is immobilized in different concentrations of sodium alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. maximum enzymatic activity of 46 was obtained in 2%w/v sodium alginate solution. investigat...

We introduce a two-dimensional lattice model of immobilization and stabilization of proteinlike polymers using grafted polymers. The protein is designed to have a specific bulk conformation reproducing a catalytic cleft of natural enzymes. Our model predicts a first order denaturing adsorption transition of free proteins. On the other hand, for an immobilized protein we observe a more gradual d...

Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

Objective(s): Enzyme immobilization via nanoparticles is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. In this study lactoperoxidase was immobilized onto silica-coated magnetite nanoparticles to improve enzyme properties in the presence of cadmium chloride as an inhibitor. Materials and Methods:  The process consists of the ...

A nanostructured biocatalytic interface reversibly gating electrochemical reactions upon chemical signals processed by immobilized enzymes was architectured. The chemical signals were converted to local interfacial pH changes causing restructuring of the stimuli-responsive polymer and switching ON-OFF the electrochemical reaction.

www.MaterialsViews.com C O M M U Nanoporous Scaffold with Immobilized Enzymes during Flow-Induced Gelation for Sensitive H 2 O 2 Biosensing N IC A By Donglai Lu , Joshua Cardiel , Guozhong Cao , and Amy Q. Shen * IO N Biosensors play indispensible roles in disease diagnosis, drug screening, and forensic applications, while nanoporous scaffolds hold an enormous potential in improving the perform...

in the present study, streptomyces gulbargensis and its mutant form, s.gulbargensis mu24, immobilized on polyurethane foam were investigated for the production of l-asparaginase using groundnut cake extract as medium. the medium with an initial ph of 8.5 was inoculated with free and immobilized cells separately and then subjected to fermentation by incubation at 40oc and shaking at 200 rev/min....