Treatment of human breast epithelial cells MCF-10F with 17-β-estradiol has been reported to result in E2-transformed cells which have given rise to highly invasive C5 cells that in turn generate tumors in SCID mice. From these tumors, various cell lines, among which C5-A6-T6 and C5-A8-T8, were obtained. Although different phases of the tumorigenesis process in this model have been studied in mo...

A new series of 1H- and 2H-pyrazole derivatives (35 final compounds) has been designed and synthesized in this study. A selected group (13 compounds) was then tested over a panel of 60 cancer cell lines at a single dose concentration of 10microM. At this concentration, six compounds have showed moderate to strong mean inhibitions, and were further tested at five-dose testing mode to determine t...

In this paper, we consider orthogonal stability of mixed type additive and cubic functional equation of the form $$f(2x+y)+f(2x-y)-f(4x)=2f (x+y)+2f(x-y)-8f(2x) +10f(x)-2f(-x),$$ with $xbot y$, where $bot$  is orthogonality in the sense of Ratz.

The human breast epithelial cell line MCF-10 was derived from s.c. mastectomy tissue from a 36-year-old, parous, premenopausal woman with fibrocystic disease. It was initiated as a mortal cell line (MCF-10M), which senesces when transferred serially in 1.05 mM calcium. These cells spontaneously gave origin to two immortal sublines, MCF-10A, or attached cells, and MCF-10F, or floating cells, whi...

Breast cancer, the most common neoplasm in women of all ages, is the leading cause of cancer-related mortality in women worldwide. Markers to help to predict the risk of progression and ultimately provide non-surgical treatment options would be of great benefit. At present, there are no available molecular markers to predict the risk of carcinoma in situ progression to invasive cancer; therefor...

The human breast epithelial cell line MCF-10 was derived from s.c. mastectomy tissue from a 36-year-old, parous, premenopausal woman with fibrocystic disease. It was initiated as a mortal cell line (Mt '!•'KIM), which senesces when transferred serially in 1.05 HIMcalcium. These cells spontaneously gave origin to two immortal sublines, IMCF10A, or attached cells, and MCF-10F, or floating cells...

Breast cancer, the most common spontaneous malignancy diagnosed in women, is a classical model of hormone dependency as it is associated with prolonged exposure to female hormones. Different cytoplasmic proteins are important in the transformation of a normal cell to an invasive tumor cell, and these include vimentin and Notch. To investigate the importance of these two genes and proteins in br...

Human tumors often display increased expression of cyclin E, suggesting that this might contribute to their abnormal growth. However, we reported recently that overexpression of a human cyclin E cDNA in the nontransformed mouse mammary epithelial cell line HC11 resulted in increased expression of the cell cycle-inhibitory protein p27Kip1 and inhibition of cell growth. To further address the rol...