When grown in the presence of bile acids, two strains of Clostridium limosum were found to contain significant amounts of NADP-dependent 7a/7/3-hydroxysteroid dehydrogenase and NAD-dependent 7a-hydroxysteroid dehydrogenase which were active against conjugated and unconjugated bile acids. No measurable activity could be found when deoxycholic acid (3a, 12a-dihydroxy-5/3-cholan-24-oic acid) was u...

INTRODUCTION Prolonged steroid treatment administered to any patient can cause visceral obesity, which is associated with metabolic disease and Cushing's syndrome. Glucocorticoids have a profound negative effect on adipose tissue mass, giving rise to obesity, which in turn is regulated by the 11β-hydroxysteroid dehydrogenase type 1 enzyme. Adrenalectomized rats treated with dexamethasone exhibi...

By selecting for growth on testosterone or estradiol17B as the only source of organic carbon, we have isolated a number of soil microorganisms which contain highly active and novel, inducible, NAD-linked 3a-, 3&, and 176-hydroxysteroid dehydrogenases. Such enzymes are suitable for the microanalysis of steroids and of steroid-transforming enzymes, as well as for performing stereoselective oxidat...

Rhodococcus equi ATCC13557 was selected as a model organism to study oestrogen degradation based on its previous ability degrade 17α-ethinylestradiol (EE2). Biodegradation experiments revealed that R. unable metabolise EE2. However, it able E2 with the major metabolite being E1 no further of E1. conversion into incomplete, 11.2 and 50.6% degraded in mixed (E1-E2-EE2) E2-only conditions, respect...

Male rats exposed to continous light for 70 days showed an increased weights of testis, accessory sex organs, serum levels of FSH, LH, testosterone, testicular 17beta-hydroxysteroid dehydrogenase (1beta-HSD) activity and alpha 2u-globulin, while 3beta-hydroxysteroid dehydrogenase activity showed no significant changes. Prolonged light exposure also stimulated sepermatogenesis in rats. These res...

Hydroxysteroid dehydrogenases (HSDHs) are from two superfamilies of short-chain dehydrogenase (SDR) and aldo–keto reductase (AKR). The HSDHs were summarized classified according to their structural functional differences. A typical pair enzymes, 7α–hydroxysteroid (7α–HSDH) 7β–hydroxysteroid (7β–HSDH), have been reported before. Molecular docking 7-keto–lithocholic acid(7–KLA) the binary 7β–HSDH...

A, androstenedione Arom, aromatase AMH, anti Müllerian hormone cAMP, adenosine 3': 5'cyclic monophosphate DHT, dihydrotestosterone E, estrogen E1, estrone E2, estradiol 17B ER, estrogen receptor FT, female-producing temperature 3B-HSD, 3B-hydroxysteroid dehydrogenase-5-ene-4-ene isomerase 17B-HSD, 17B-hydroxysteroid dehydrogenase hsp, heat-shock protein MT, male-producing temperature P 450 arom...

We found that 3α-hydroxysteroid dehydrogenase (3α-HSD) from Pseudomonas sp. B-0831 can catalyze the carbonyl reduction of non-steroid substrates, such as metyrapone and p-nitrobenzaldehyde, in addition to the activity of 3α-hydroxysteroid dehydrogenase. The results of an inhibition study in metyrapone reduction by androsterone and cholic acid indicated that metyrapone bound to the same catalyti...

Tetrahydrobiopterin (BH(4)) is a cofactor for aromatic amino acid hydroxylases and nitric oxide synthase. The biosynthesis includes two reduction steps catalyzed by sepiapterin reductase. An intermediate, 6-pyruvoyltetrahydropterin (PPH(4)) is reduced to 1(')-oxo-2(')-hydroxypropyl-tetrahydropterin (1(')-OXPH(4)) or 1(')-hydroxy-2(')-oxopropyl-tetrahydropterin (2(')-OXPH(4)), which is further c...