To digitally analyze expression levels of multiple genes in one reaction, we proposed a method termed as 'MDHB' (Multiplexed Digital-PCR coupled with Hydrogel Bead-array). The template for bead-based emulsion PCR (emPCR) was prepared by reverse transcription using sequence-tagged primers. The beads recovered from emPCR were immobilized with hydrogel to form a single-bead layer on a chip, and th...

Encoded combinatorial library technologies have dramatically expanded the chemical space for screening but are usually only analyzed by affinity selection binding. It would be highly advantageous to reformat outputs ”one-bead-one-compound” solid-phase libraries, unlocking activity-based and cellular capabilities. Here, we describe hydrogel-encapsulated magnetic beads that enable such a transfor...

PURPOSE Evaluation of doxorubicin stability over time when stored into the DC Bead embolic agent, in various containers, which are used for the delivery of the doxorubicin-loaded beads to the patients for up to 14 days under refrigerated conditions. METHODS The doxorubicin was loaded through the ionic exchange mechanism into the calibrated polyvinyl alcohol-based hydrogel beads (DC Bead), wit...

This paper describes a selective retrieval method for arrayed monodisperse hydrogel beads containing cells. We implemented modifications such as: (i) the incorporation of cavities as nucleation sites, (ii) indirect retrieval using bubble powered jets and (iii) the use of low boiling point fluid in our device to realize a gentle optical-based retrieval method. Parametric studies confirmed that t...

Transcriptional profiling, which is directly or indirectly associated with expressed protein levels, has been used in various applications including clinical prognosis and pharmaceutical investigation of drug activities. Although the widely used reverse transcription polymerase chain reaction (RT-PCR) allows for the quantification of absolute amounts of mRNA (mRNA) from inputs as small as a sin...

Understanding the intra-tumoral distribution of chemotherapeutic drugs is extremely important in predicting therapeutic outcome. Tissue mimicking gel phantoms are useful for studying drug vitro but quantifying laborious due to need section over relevant time course and individually quantify elution. In this study we compare a bespoke version traditional phantom sectioning approach, with novel c...

Adsorption and water treatment of Ca (II) and Mg (II) hardness were investigated via adsorption of metal ions onto commercial polyacrylic acid hydrogel beads as a novel sorbent for metal ions (Ca2+, Mg2+) removal and water treatment. Batch equilibrium technique was carried out under the influence of solution pH, contact time, sorbent dosage, initial metal concentration and...

Adsorption and water treatment of Ca (II) and Mg (II) hardness were investigated via adsorption of metal ions onto commercial polyacrylic acid hydrogel beads as a novel sorbent for metal ions (Ca2+, Mg2+) removal and water treatment. Batch equilibrium technique was carried out under the influence of solution pH, contact time, sorbent dosage, initial metal concentration and...

a Inson Medical Systems, Inc, Bellevue, WA 98005 U.S.A., Department of Ophthalmology, University of Washington, Seattle, WA 98195 U.S.A., Department of Bioengineering, University of Washington, Seattle, WA 98195 U.S.A. Introduction: Cataract is the leading cause of treatable blindness worldwide [1], and the population afflicted with cataract is increasing. The primary treatment for cataracts is...

DC Bead is a drug delivery embolisation system that can be loaded with doxorubicin or irinotecan for the treatment of a variety of liver cancers. In this study we demonstrate that the topoisomerase I inhibitor topotecan hydrochloride can be successfully loaded into the DC Bead sulfonate-modified polyvinyl alcohol hydrogel matrix, resulting in a sustained-release drug eluting bead (DEBTOP) usefu...