Hydatid disease caused by Echinococcus granulosus is a zoonotic infection of cosmopolitan distribution. As the clinical manifestations of hydatid disease in man are variable, the diagnosis of the condition presents complex problems for clinicians. Since the parasitic diagnosis of the disease is difficult, the specific diagnosis of the condition relies heavily on immunodiagnostic tests. The rece...

Njeruh, F.M; Gathuma, J.M; Tumboh-Oeri, A.G Date: 1986 A method for preparation of a hydatid cyst antigen antigen "880" of Echinococcus granulosus origin by heat treatment of concentrated cattle hydatid cyst fluid (HCF) is described. The thermo-stable antigen was applied in the serological diagnosis of human hydatidosis where a total of 78 "blind" sera were examined by the indirect hemagglutina...

background : the current study was designed to evaluate immune responses induced by dna vaccines encoding 8-kda subunit of antigen b (hydi) of echinococcus granulosus and murine interleukin 12 (il-12) as genetic adjuvants in balb/c mice. methods : expression plasmid pcdna3.1 containing hydi (pchyd1) as vaccine along with the murine interleukin 12 (pcmil12) as adjuvant were used. thirty-five mic...

hydatid cyst is a world wide and zoonotic disease between human and animal. in this study 384 buffalos were used for blood sampling according to cocran formula. in compliance with elisa kit to study the samples, the needed antigen was removed from the fertile hydatid cysts and purified according to oriol and rogan method.then reliability of the conjugate (biodesign company) required density of ...

Spleen is an unusual location for hydatid cyst. Here we report a case of primary splenic hydatid cyst in a 41-yr-old Iranian woman from Yasuj, southwest of Iran. The patient had been admitted to Shahid Beheshti Hospital because of abdominal pain. Abdominal sonography revealed a hypoechoic lesion of 150 X 130 mm in the spleen, suggestive of hydatid cyst. Splenectomy was performed for the patient...

Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), c...

The current work aimed to evaluate the cross-reactivity of human immune sera against crude hydatid fluid antigens of sheep, human, mouse, cattle, as well as B fraction of cystic fluid antigen. 30 balb/c mice were infected with sheep hydatid cyct fluid antigen containing protoscolex after the viability of these protoscolices was assessed. ANOVA was used to test the difference of themean of optic...

Human 0 erythrocytes, treated with 1% formalin and subsequently exposed simultaneously to hydatid protein antigen and 0.1% chromic chloride, acquire the property of agglutinating on a slide when brought in coontact with specific antiserum. This constitutes the basis of the slide haemagglutination test, an easy, quick, sensitive, and specific procedure, usefull in the diagnosis of hydatid disease.

BACKGROUND Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensitive, however their specificity are not satisfactory. Therefore, WHO recommended specific serological methods using specific antigens, sp...