نتایج جستجو برای: hybridization
تعداد نتایج: 54161 فیلتر نتایج به سال:
many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. in this study for asessing genetic diversity among bradyrhizobium japonicum isolates, two different probes (suca and topa) chosen from the chromosomal genome of bradyrhizobium strain usda 110 were designed, evaluated by dnaman software and im...
دورگه سازی در محل، روشی است که در آن از پروب های اسید نوکلئیک برای بررسی انواع تغییرات ژنتیکی در سلول های دست نخورده و بافت های تثبیت شده استفاده می شود. مراحل مختلف این روش شامل انتخاب پروب، آماده سازی نمونه، تیمار پیش از دورگه سازی، دورگه سازی، شستشو، آشکارسازی و روند کنترل می باشند. انتخاب پروب مناسب یکی از جنبه های مهم انجام فرایند دورگه سازی موفقیت آمیز است. حساسیت و ویژگی in situ hybridiz...
Twenty to thirty percent of causative agents of the respiratory tract infections are still unknown, that can be related to the lack of suitable laboratory procedures. Some of these organisms may cause progressive infection such as encephalitis and cardiac complications. To recognize the M. pneumoniae , PCR-hybridization technique was used and a specific internal probe was produced for this...
در این تحقیق ارزیابی آلودگی ارقام تجاری پرتقال تامسون ناول و خونی مورو، نارنگی پیج و پوملو وبر نسبت به ویروئیدهای مرکبات مورد بررسی قرار گرفت. بر این اساس گیاه بالنگ اتراگ (c. medica) به عنوان محک عمومی ویروئید های مرکبات با اینوکولوم از منشا درختان مادری ارقام مذکور از طریق پیوند آلوده شده و در شرایط کنترل شده دمایی گلخانه (روز40، شب28 درجه سانتی گراد) نگهداری گردید. پس از آلوده سازی استخراج ا...
background and aims: viroids are smallest known plant pathogens and cause several economically significant diseases. until recently, viroid detection relied mainly on biological tests and indexing. today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (rt-pcr) are being used for detection and diagno...
in order to produce new hybrids and evaluate the general and specific combining ability of sunflower inbred lines and restorers, four inbred lines and six restorers were selected in 2004. twenty-four f1 hybrids were derived in 2004 and evaluated on a rcbd design with three replications in 2005. the results of variance analysis showed that treatments were significantly different for all traits e...
An Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays
Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules. Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...
in this thesis a calibration transfer method is used to achieve bilinearity for augmented first order kinetic data. first, the proposed method is investigated using simulated data and next the concept is applied to experimental data. the experimental data consists of spectroscopic monitoring of the first order degradation reaction of carbaryl. this component is used for control of pests in frui...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید