Propose: Standard electroporation and electrochemotherapy caused the endothe- lial cell permeability and reduction in tumor blood flow. The effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. Therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and perme...

inducible nitric oxide synthase (inos) gene expresses a calcium calmudolin-independent enzyme which can catalyse no production from l-arginine. the induction of inos activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (lps). previous studies indicated that all nitric oxide synthases (nos) activated in human umbilical vein endot...

propose: standard electroporation and electrochemotherapy caused the endothe- lial cell permeability and reduction in tumor blood flow. the effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and perme...

BACKGROUND AND OBJECTIVES Clotting activation and thromboembolic manifestations are common features in patients with cancer. The two-way interaction between tumor cells and host cells is of crucial importance in this context. In the present study we investigated the effect of tumor cell-endothelial cell co-culture on the expression of procoagulant activity in the mixed cell populations. DESIG...

T cell adhesion to endothelium is critical to lymphocyte recirculation and influx into sites of inflammation. We have systematically analyzed the role of four receptor/ligand interactions that mediate adhesion of peripheral human CD4+ T cells to cultured human umbilical vein endothelial cells (HUVEC): T cell LFA-1 binding to ICAM-1 and an alternative ligand ("ICAM-X"), T cell VLA-4 binding to V...

Damage and inflammation of endothelial cells are important factors in the pathogenesis of atherosclerosis. Shear and NAD-dependent protein sirtuin-4 (SIRT4) might regulate atherosclerosis through endothelial cells. This study will investigate the mechanism of how shear and SIRT4 regulate human umbilical vein endothelial cells (HUVEC) to affect atherosclerosis. HUVEC were isolated, cultured, and...

introduction: proteolytic enzymes, specially collagenase, are used to digest extracellular matrix, cells isolation and primary culture. it is important to find new sources of plant or animal protease instead of bacterial or tissue collagenase. in the present research, actinidin, a plentiful protease in kiwifriut, was used to isolate human umbilical vein endothelial cells. materials and methods:...

Proinflammatory stimuli lead to endothelial injury, which results in pathologies such as cardiovascular diseases, autoimmune and contributes alloimmune responses after organ transplantation. Both mesenchymal stromal cells (MSC) the extracellular vesicles (EV) released by them are widely studied regenerative therapy for endothelium. However, therapeutic application, manipulation of living MSC la...

BACKGROUND AND PURPOSE Agents that protect against neurovascular damage provide a powerful neuroprotective strategy. Human umbilical vein endothelial cells (HUVECs) may be used to treat neonates with hypoxic-ischemia (HI) because of its autologous capability. We hypothesized that peripherally injected HUVECs entered the brain after HI, protected against neurovascular damage, and provided protec...

OBJECTIVES To search for more effective radiation protectors with minimal toxicity, a water-soluble nitroxides Acetamido-Tempol (AA-Tempol) was evaluated for potential radioprotective properties in HUVEC cells (Human Umbilical Vein Endothelial cell line). METHODS To study the anti-radiation effect of AA-Tempol in cell culture, the viability of irradiated HUVEC cells using a clonogenic surviva...