Epimers of ginsenoside Rg3 (Rg3) have a low bioavailability and are prone to deglycosylation, which produces epimers Rh2 (S–Rh2 R–Rh2) protopanaxadiol (S-PPD R-PPD). The aim this study was compare the efficacy potency these molecules as anti-cancer agents. Crystal violet staining used anti-proliferatory action on human epithelial breast cancer cell line, MDA-MB-231, umbilical vein endothelial c...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 7 975–986 www.jcb.org/cgi/doi/10.1083/jcb.201408103 JCB 975 Correspondence to Martin A. Schwartz: [email protected] Abbreviations used in this paper: EGCS, endothelial cell growth supplement; HUVEC, human umbilical cord endothelial cell; Ncad, N-cadherin; OSS, oscillatory shear stress; SFK, Src family kinase; TMD, transme...

objective(s):although previous studies have confirmed the beneficial effects of human umbilical cord matrix stem cell (hucm) transplantation post myocardial infarction (mi), but this stem cell resource has no potential to induce angiogenesis. in order to achieve the process of angiogenesis and cardiomyocyte regeneration, two required factors for cardiac repair agents were examined namely; hucm ...

OBJECTIVE Endothelial-colony forming cells (ECFCs) can be readily expanded from human umbilical cord blood and can facilitate repair of endothelial injury. E-selectin and SDF-1α are produced following endothelial injury and can regulate endothelial progenitor homing. Mechanisms of vascular repair specific to the mode of injury have not been well described in homogenous cell populations such as ...

Recent studies in our laboratory, as well as others, have suggested that fibrin can regulate cell function in vitro and likely control inflammation in vivo by acting as a potent cell activator. This has led us to hypothesize that during tissue and vascular injury, fibrin can enhance leukocyte recruitment by inducing vascular endothelial cell expression of leukocyte chemotactic factors. To begin...

The accumulation of leukocytic infiltrates in perivascular tissues is a key step in the pathogenesis of Lyme disease, a chronic inflammatory disorder caused by Borrelia burgdorferi. During an inflammatory response, endothelial cell adhesion molecules mediate the attachment of circulating leukocytes to the blood vessel wall and their subsequent extravasation into perivascular tissues. Using cult...

The biologic activity of C1 esterase, activated forms of factor XII and kallikrein at sites of vascular inflammation may be regulated by C1 inhibitor (C1 INH) elaborated by endothelial cells. Therefore, we investigated whether human umbilical vein endothelial cells (HUVEC) in culture produce C1 INH. Passaged HUVEC contain 1.6 +/- 0.8 micrograms of C1 INH/10(8) cells (mean +/- S.D.; n = 7) which...

Human umbilical vein endothelial cell (HUVEC) isolated from umbilical cord is widely used as endothelial cell model. However, HUVEC has been characteristically hard to maintain and showed molecular heterogeneity depending on the umbilical cord donors. Commercial HUVEC is commonly derived from European and Caucasian population which have different molecular characteristics from Asian women. This...

BACKGROUND Endothelial Progenitor Cells (EPC) support neovascularization and regeneration of injured endothelium both by providing a proliferative cell pool capable of differentiation into mature vascular endothelial cells and by secretion of angiogenic growth factors. OBJECTIVE The aim of this study was to investigate the role of PDGF-BB and PDGFRβ in EPC-mediated angiogenesis of differentia...

objective: immunotoxins (its) have been developed for the treatment of cancer, and comprise of antibodies linked to toxins. also vascular endothelial growth factor (vegf) plays a key role in tumor angiogenesis, and the blockade of vegf receptor-2 (vegfr2) inhibits angiogenesis and tumor growth. the aim of this study was to produce anti-vegfr2/rpe (pseudomonas exotoxin) 38 it to test its cytotox...