This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR. Supplementary semi-nested human metapneumovirus and picornavirus PCR assays were required to complete the acute respiratory pathogen profile. P...

transmission of human pathogens can be occurred via inert objects. paper currency is a further common contact surface whereby pathogens can be transferred within a population although the significance remains unknown. hence, the aim of the present study was to investigate microbial populations associated with iranian paper currency.this study was carried out by getting 108 samples of the irania...