background:   objective: in this study, we supplemented a chemically defined medium with different combinations of selenium, calcium and calcium ionophore concentrations to obtain the best rate of human oocytes maturation, survival, and fertilization.   materials and methods: as an experimental study, three combinations of [selenium (5 μg/ml), calcium (5 μg/ml) and calcium ionophore (1 μg/ml)],...

Background: The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. The zona pellucid (ZP) is a dynamic matrix composed of filaments with the properties that might reflect the history of oocyte cytoplasmic maturation. Since, spindle and ZP in living oocytes are highly birefringent, their structures can be viewed noninvasively by using a P...

Objective:In vitro maturation is a highly effective method for achieving mature  oocytes to treat infertility based on in vitro methods (IVF-ICSI). This technique is effective for treating women who suffer from polycystic ovary syndrome (PCO) and cancer. MATERIALS AND METHODS: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units of...

background: in general, 15% of oocytes collected in art cycles are immature. these oocytes may be cryopreserved further for use in in-vitro maturation (ivm) program. objective: the aim of this study was to determine maturation capacity, morphometric parameters and morphology of human immature oocytes in both fresh ivm (fivm) and vitrified-ivm (vivm) oocytes. materials and methods: 93 women who ...

Medical treatments at the beginning and end of human life are highly contested in public discourse. Our study reveals factors shaping acceptance social egg freezing (or oocyte cryopreservation) as an assisted reproduction technology (ART) general public. Based on theory moral contextualism literature medical ethics, we deduce potential influencing attitudes toward cryopreservation (for example,...

Objectives: The frequency of aneuploidies in human oocytes is extremely high. It is hypothesized that the cause may be due to abnormal chromatin (histone) acetylation/deacetylation. The aim of our study was to analyzed the acetylation/deacetylation pattern in spare human oocytes. Materials and Methods: Human spare oocytes (311), in other words oocytes that were not mature when collected from fo...

The purpose of this study was to evaluate the effectiveness cysteamine supplementation on maturation medium and/or in vitro fertilization with regards improving normal rate sheep oocytes, which are characterized by formation two pronuclei. Grade A and B oocytes were matured medium-199 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), human chorionic gonadotrophin (hC...

STUDY QUESTION Does the position of the germinal vesicle (GV) in human oocytes correlate with molecular and morphological parameters as well as with maturation-competence? SUMMARY ANSWER The position of GV in human oocytes correlates with density of microtubule (MT) filaments, concentration of Fyn, nucleolus localization and the ability of the oocytes to complete maturation following GV break...

BACKGROUND Mouse oocytes can be used in conjunction with intracytoplasmic sperm injection (ICSI) as a technique to permit chromosomal analysis of human sperm. However, chromosomes derived both from the human sperm and the mouse oocyte appear simultaneously following ICSI. The present study focused on evaluating whether or not previously enucleated mouse oocytes are usable for the analysis of hu...

background: artificial stimulation of mouse oocyte, in the absence of sperm contribution, can induce its parthenogenic activation of oocyte. ultrasound is one of the newest methods for artificial activation of mammal oocytes, and its successful utilization in pig oocyte activation has been recently reported. our objective was to assess the effect of ultrasound on mouse oocyte activation. materi...