Human growth hormone (hGH) bound to specific sites on rat hepatocytes. The time course of hGH dissociation was comprised of more than one component. Dissociation was resolved into rapid ( t l l z = 10.5 min) and slow (tilz = 6.4 h) fractions. The amount of slowly dissociable hormone increased for the first 75 min during which time cells and [Iz5I]hGH associated. Subsequently, the amount of slow...

Thirty-six children with growth hormone deficiency were treated for up to 48 months with methionyl human growth hormone (hGH) synthesised by DNA recombinant methods. The growth rate for these children increased from 3.2 +/- 1.1 cm/yr to 10.5 +/- 2.2 cm/yr (mean +/- SD). This was similar to the effect of pituitary hGH in ten GH deficient children, 3.8 +/- 1.0 to 10.1 +/- 1.1 cm/yr. Serum somatom...

Two endocrine functions, HGH synthesis and release, were separately studied on human chromophobe adenomas, isolating the hormone with polyacrylamide gel disc electrophoresis after in vitro labelling of the hormone with 14C-leucine. The results of the experiments utilizing fresh adenoma tissues obtained from 8 cases at operation revealed that all of the adenomas synthesized and...

We have sought direct evidence for the in vivo expression of the human growth hormone-variant (hGH-V) gene by screening a placental cDNA library with a hGH-V-specific oligonucleotide. Nine independent hGH-V cDNA clones were isolated and analyzed, and three distinct species were detected. Five of these hGH-V cDNAs represent mRNAs spliced and processed in a pattern analogous to that of the highly...

human growth hormone (hgh) is a protein with multiple roles in a range of biological functions such as protein, carbohydrates and lipid metabolisms as well as immunity, tissue development and overall growth. one of the major class of biopharmaceuticals in mammalian cells is the production of recombinant pharmaceutical proteins. in this study, we constructed a lentiviral vector carrying coding r...

Human growth hormone (hGH) gene expression and regulation by thyroid and glucocorticoid hormones were examined after its stable introduction into rat anterior pituitary tumor cells. Transcripts from the hGH gene were correctly initiated and could be positively regulated by glucocorticoids. In contrast to the endogenous rat GH gene and a transfected, modified rat GH gene, the hGH gene was negati...

Brook, C. G. D. (1973). Archives of Disease in Childhood, 48, 725. Effect of human growth hormone treatment on adipose tissue in children. Body fat and the size and number of adipose cells were determined in 22 children with growth hormone deficiency before and after 6 months of replacement therapy with human growth hormone (HGH). A marked reduction in body fat and adipose cell size was found, ...

The anterior pituitary gland produces a 20-kilodalton (kDa) variant of human growth hormone (hGH) that differs from the predominant 22-kDa form of hGH in that amino acid residues 32-46 are deleted. Previous work has suggested that the 20-kDa variant possesses the full growth-promoting and lactogenic activities of 22-kDa hGH but lacks its intrinsic diabetogenic and insulin-like activities. In th...