In this study, the structural characters, antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides (HRPs) obtained by commonly used hot water (HRP-W), pressurized (HRP-H), ultrasonic (HRP-U), acid (HRP-C) alkali (HRP-A) assisted extraction methods were investigated. The results demonstrated that had significant effects on yield, monosaccharide composition, molecular...

PURPOSE Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. METHODS Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse IgG2b. Sodium citrate buffer (0.1 M, pH: 3.5) was used for separation of mouse IgG2b. Verification of the purified fractions was monitored by S...

Herein, we report preparation of magnetic hybrid nanoflower (MhNF) formed horseradish peroxidase (HRP), copper ions (Cu2+) and iron oxide nanoparticles (Fe3O4 NPs) with greatly enhanced activity, stability, reusability applicability for use in removal phenol from aqueous solution. In this study, pre-synthesized small sized Fe3O4 NPs were immobilized to synthesized HRP-Cu2+ (hNFs). The MhNFs cha...

To design an enzyme immunoassay-based method, the most important is conjugation of enzyme with antigens or antibodies and stability of labeled conjugate. In this study, human albumin was conjugated with horseradish peroxidase using periodate oxidation method. To stabilize biological activity and functional structure of different components of protein conjugate, special storage conditions are re...

A simple colorimetric immunoassay system, based on the peroxidase mimicking activity of Fe3O4 magnetic nanoparticles (MNPs), has been developed to detect clinically important antigenic molecules. MNPs with ca. 10 nm in diameter were synthesized and conjugated with specific antibodies against target molecules, such as rotaviruses and breast cancer cells. Conjugation of the MNPs with antibodies (...

Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red the most widely used substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular improved sensitivity and selectivity HRP thus desired. Herein, three dihydroflu...

An emerging trend in utilizing service robots a vast range of application areas could be seen nowadays as promising effort to uplift the living standard. These are intended used by non-expert users, and their tasks often require navigation human-populated environments. Thus, human-friendly behavior is expected from these users. A robot should aware Human–Robot Proxemics (HRP) facilitate behavio...

The binding behavior of a competitive inhibitor, N-phenylbenzhydroxamic acid (BHA) against horseradish peroxidase (HRP) was studied in order to understand and predict the interaction mechanism of hydrogen donors with the enzyme. The dissociation constants of the complexes of HRP-BHA, HRP-donor and HRP-BHA-azide were estimated at specified conditions by difference spectroscopy. The binding s...

Undiluted urine mixed directly with HRP suppresses generation of enzyme product to less than 80% of that seen in buffer controls. Incubating dilutions of various urine preparations with HRP immobilized on concanavalin A coated microtiter plates reveals that the source of urine or HRP, and the type of HRP substrate used have minimal effect on the degree of HRP suppression; only dilutions of urin...