The rabbit is a classical experimental animal species. A major limitation in using rabbits for biomedical research is the lack of germ-line-competent rabbit embryonic stem cells (rbESCs). We hypothesized that the use of homologous feeder cells and recombinant rabbit leukemia inhibitory factor (rbLIF) might improve the chance in deriving germ-line-competent rbES cells. In the present study, we e...

Uncertainty propagation in a level 2 high level information fusion (HLIF) process is affected by a number of considerations. These include the varying complexities of the various types of level 2 HLIF. Five different types are identified, ranging from simple entity attribute refinement using situation status data to the development of a complete situation assessment assembled from applicable si...

Embryonic stem cells (ESCs) are originally derived from the ICM of blastocysts and are characterized by their ability to self-renew and their pluripotencies. Only a few reports have been published on ESC isolations and line establishment in animals, even fewer in horses. However, it is still important to isolate equine ESCs for animal biotechnology and therapeutic applications. In the present s...

Author's Current Address: E.P. BIasch, Air Force Research Laboratory, NY, USA; D.A. Lambert, Defence Science & Tech. Organization, Australia; P. Valin, Defence R&D Canada Valcartier; M. "Mitch" M. Kokar, Northeastern University, MA, USA; J. Llinas, SUNY Buffillo, NY, USA; S. Das, Machine Analytics, MA, USA; C. Chong, BAB Systems, CA, USA; and E. Shahbazian, OODA Technologies, Canada. Manuscxrip...

Melanoma is the deadliest form of skin cancer. With rising incidence rates and subjectivity in current clinical detection methods, there is a need for melanoma decision support systems. The HLIFs were designed to model the ABCD criteria commonly used by dermatologists such that each HLIF represents a human-observable characteristic. As such, intuitive diagnostic rationale can be conveyed to the...

embryonic stem cells (escs) are originally derived from the icm of blastocysts and are characterized by their ability to self-renew and their pluripotencies. only a few reports have been published on esc isolations and line establishment in animals, even fewer in horses. however, it is still important to isolate equine escs for animal biotechnology and therapeutic applications. in the present s...

The proliferation rate and differentiation state were investigated in porcine inner cell masses (ICMs) and epiblasts in vitro. ICMs isolated from early blastocysts (Day 7 of pregnancy) and epiblasts isolated from preelongated blastocysts (Day 11 of pregnancy) were cultured for up to 5 days in the presence of human leukemia inhibitory factor (hLIF) (1000 U/ml). The proliferation rate was evaluat...

While culture conditions for embryos have improved, development in vitro is not equivalent to in vivo growth. Platelet-derived growth factor (PDGF), mouse (mLIF) or human (hLIF) leukemia inhibitory factor and 10% fetal bovine serum (FBS) added to protein-free culture medium, as well as two gas atmospheres were evaluated for their effect on rabbit embryo development. Adding PDGF, mLIF or hLIF to...

Embryonic stem (ES) cells with the capacity for germ line transmission have only been verified in mouse and rat. Methods for derivation, propagation, and differentiation of ES cells from domestic animals have not been fully established. Here, we describe derivation of ES cells from goat embryos. In vivo-derived embryos were cultured on goat fetal fibroblast feeders. Embryos either attached to t...