We have developed a quantitative histochemical assay for measurement of succinate dehydrogenase (SDH) activity in single motoneurons. A computer image processing system was used to quantify the histochemical enzyme reaction product and to follow the time course of the reaction. The optimal concentration for each of the ingredients of the incubation medium for the SDH reaction was determined and...

Background: Infertility problem affect more than 15% of young couples in different societies. One of the known causes of ovulation disorder is chemotherapy in patients with cancer. This side effect may last from ten years up to the end of the life. Since anticancer drugs are mainly insert their effect through decreasing of cell proliferation, the aim of the present study is to investigate, anti...

This is the first study devoted to the histologic and histochemical characteristics of the orbicularis oculi muscle in children to the authors' knowledge. The orbicularis muscle was compared with extraocular, facial, and limb striated muscle. Light microscopy showed the orbicularis oculi muscle to be much smaller and more loosely packed than skeletal limb muscles. It further showed these muscle...

Recently, the salivary glands function from biochemical, physiological and histochemical standpoints have been rather highlighted. Many investigators have come to try a histochemical detection of enzymes participating in various metabolic pathway in the salivary glands of experimental animals and humans. The previous study of this series dealt with the histochemical demonstration of various hyd...

In general, for the histochemical demonstration of peroxidase , b enzidine, naphthol, and leuco-dyes are utilized in the prosence of hydrogen peroxide. Among these , the technical modifications of the benzidine-peroxide method are the most numerous . Alpha-naphthol has been used less often than benzidine . Application of the leucodye method for animal tissues is remarkably limited and this meth...

Lectins have been intensively used in histochemical techniques for cell surface characterization. These proteins are involved in several biological processes and their use as histochemical markers have been evaluated since they can indicate differences in cell surfaces. Parkia pendula lectin (PpeL) was evaluated as histochemical marker for meningothelial meningioma biopsies. Tissue slices were ...

Histochemistry provides the unique opportunity to detect single molecules in the very place where they exert their structural roles or functional activities: this makes it possible to correlate structural organization and function, and may be fruitfully exploited in countless biomedical research topics. Aiming to estimate the impact of histochemical articles in the biomedical field, the last fe...