L-Phenylalanine ammonia-lyase has been purified from elicitortreated alfalfa (Medicago sativa L.) cell suspension cultures using two protocols based on different sequences of chromatofocusing and hydrophobic interaction chromatography. Three distinct forms of the intact enzyme were separated on the basis of affinity for Octyl-Sepharose, with isoelectric points in the range pH 5.1 to 5.4. The na...

Phenylalanine ammonia-lyase (PAL) performs a major role in the biosynthesis of polyphenolic compounds, which are involved in the defense mechanism in harsh environments related to different stimuli. The present study was carried out to detect the activity of phenylalanine ammonia-lyase in different plant parts of Anacardium occidentale such as flower, young leaves, shoot tip, mature leaves, raw...

Glyphosate showed a remarkable effect inducing the change of flower symmetry from the actinomorphic to the zygomorphic type in Petunia hybrida. Glyphosate [N-(phosphonomethyl)glycine] reduced the anthocyanin content and showed a weak inhibitory effect against phenylalanine ammonia-lyase (PAL) activity. L-2-Aminooxy-3-phenylpropionic acid (APA), an inhibitor of PAL activity, reduced the anthocya...

Scape bending disorder is the most important factor affecting postharvest loss of gerbera cut flowers. One of the ultimate reasons for gerbera stem bending is lignin, with deformation structural functions and defensive mechanisms. This postharvest experiment was conducted to evaluate the role of phenylalanine ammonia lyase (PAL) and peroxidase (POD) enzymes activity in stem bending of two gerbe...

Wheat (Triticum aestivum L. cv Fremont) grown in hydroponic culture under 24-hour continuous irradiation at 560 to 580 micromoles per square meter per second from either metalhalide (MH), high pressure sodium (HPS), or low pressure sodium (LPS) lamps reached maturity in 70 days. Grain yields were similar under all three lamps, although LPS-grown plants lodged at maturity. Phenylalanine ammoni...

Biosynthesis of phenylpropanoid natural products in tobacco was perturbed by introduction of a heterologous (bean) phenylalanine ammonia-lyase (PAL; L-phenylalanine ammonia-lyase, EC 4.3.1.5) gene, modified by inclusion of cauliflower mosaic virus 35S enhancer sequences in its promoter. These transgenic plants can exhibit a series of unusual phenotypes including localized fluorescent lesions, a...

Plant growth and activity of peroxidases (POX) and phenylalanine ammonia-lyase (PAL) were investigated in seedlings of yellow lupine (Lupinus luteus L.) and soybean (Glycine max L.) treated with copper (25 mg/l) and an inhibitor of ethylene biosynthesis (CoCl 2 ). Both lupine and soybean plants stressed with an excess of copper exhibited a decrease in root length and fresh weight. In lupine pla...

Aromatic ammonia-lyases catalyze the non-oxidative ammonia elimination from aromatic Lamino-acids, producing α,β-unsaturated compounds. The members of this enzyme family are the phenylalanine ammonia-lyase (PAL, EC 4.3.1.24), (Hodgins, 1971) histidine ammonia-lyase (HAL, EC 4.3.1.3) (Givot et al., 1969; Wickner, 1969) and the tyrosine ammonia-lyase (TAL, EC 4.3.1.23) (Kyndt et al., 2002) cataly...

effects of pre-storage application of salicylic acid (sa) and storage periods were investigated on phenylalanine ammonia lyase (pal) activity, chilling index (ci) and quality of pomegranate (punica granatum) cv. malase saveh. the results showed that with increase in sa concentration up to 2mm, ci and ion leakage (il) were reduced while pal activity being increased. the lower il and ci vc the hi...

the most important postharvest disorder of cut gerbera is stem bending disorder, which occurs below the capitulum. stem strength and lignification is one of important phenomenon and causes of gerbera stem bending. therefore, this experiment was conducted to evaluate the effects of salicylic acid (sa) on phenylalanine ammonia-lyase enzyme activity (pal), as an important enzyme in phenylpropanoid...