An isocratic reversed-phase high-pressure liquid chromatographic method for the determination of six components of the teicoplanin complex in biological fluid was developed. By using fluorescence detection after precolumn derivatization with fluorescamine, the assay is specific and highly sensitive, with reproducibility studies yielding coefficients of variation ranging from 1.5 to 8.5% (at 5 t...

background: genes for human epidermal growth factor receptors b1 (erbb1) and b2 (erbb2) were amplified in breast and ovarian cancers. both of them were associated with aggressive disease and worse prognosis. the erbb1 or erbb2 status of a tumor may provide an indication of the response to erbb1 and erbb2 -targeted therapies. for accurate and rapid assessment of amplification of erbb1 and erbb2 ...

A new model for the vapor phase of a pressurized liquid called "the cluster model: which is originally introduced in this work, along with an accurate equation of state for the liquid phase called the LIR, is used to derive an accurate equation for the vapor pressure of liquid as a function of external pressure. The chemical Potential of both phase have been found to be in good agreement wi...

The high-pressure liquid chromatographic separation of ail of the known metabolites of vitamin D2 and vitamin D3 found in biological fluids has been achieved. This technique has been successfully applied to the analysis of vitamin D mixtures, purification of vitamin D metabolites, and identification of radioactive peaks. Some theoretical bases for the observed resolutions are suggested. Supplem...

Two single-stranded nucleic acid-binding proteins, UP1 and UP2, that were originally reported by Herrick and Alberts (Herrick, G., and Alberts, B. (1976) J. Biol. Chem. 251,2124-2132) have been purified to apparent homogeneity from calf thymus by high performance liquid chromatography. The amino acid sequence of UP1 (Williams, K. R., Stone, K. L., LoPresti, M. B., Merrill, B. M., and Planck, S....