OBJECTIVES To describe the levels of exposure to fungal alpha-amylase in British bakeries and flour mills, and to describe the relation between exposure to alpha-amylase and sensitisation to fungal alpha-amylase. METHODS 495 personal flour dust samples were taken in seven British bakeries and flour mills and analysed for alpha-amylase with an immunoassay. Workers at the sites were asked to fi...

Wheat (Triticum aestivum) RNA was used to program synthesis of the alpha-amylase protein by Xenopus laevis oocytes. A 41,500-dalton protein was made which was identified as alpha-amylase by immunoprecipitation with rabbit anti-alpha-amylase antiserum raised against the purified wheat protein and by its co-migration with authentic alpha-amylase on sodium dodecyl sulfate polyacrylamide gels. Synt...

alpha-Amylase is a starch cleaving enzyme often used in the baking industry as a flour additive. It is usually of fungal origin, produced by Aspergillus oryzae. One previous report has shown IgE antibodies and positive skin prick test against alpha-amylase in asthmatic bakers. This paper describes four alpha-amylase sensitised index cases with occupational asthma or rhinitis and the results of ...

bilberry [vaccinium myrtillus linn. (ericaceae)] fruit (vmf) has been traditionally used for treatment of bladder stones, biliary disorders, scurvy, coughs, and lung tuberculosis. vmf may have some pharmaceutical properties owing to these uses, but in vivo and in vitro studies are limited for clarification of medicinal activity and its antidiabetic and antioxidant properties are not investigate...

Protein activation of urinary alpha-amylase (EC 3.2.1.1) activity was observed during an evaluation of the Du Pont aca procedure for the determination of urinary alpha-amylase. This activation effect became constant for urinary albumin concentrations exceeding 1.50 g/liter. It is recommended that urinary alpha-amylase be analyzed with sufficient albumin added to maximize this effect. The aca al...

In response to a phytohormone, gibberellic acid, the aleurone layers of barley seeds synthesize and secrete alpha-amylases, which are coded by a set of stable mRNAs. When aleurone layers are subjected to heat shock treatment, the synthesis of alpha-amylase is suppressed while heat shock proteins are induced. The suppression of alpha-amylase synthesis is not the result of translational control a...

Results Female 6 years old, previously healthy, 3-year evolution gastric recurrent vomiting , abdominal pain intermittently mesogastrio, adding hiporexia, jaundice and increased waist circumference, with palpable mass in the right upper quadrant. Cholestatic syndrome, 857 Lipase, amylase 137 Immunoglobulin IgG subclass 4: 23mg/dL (5-6 years: 1-121 ). CT: dilatation of intra and extra hepatic bi...

Background: Diabetes mellitus is a chronic disease. Decreasing postprandial hyperglycemia by retarding glucose absorption through inhibiting carbohydrates digesting enzymes (α-amylase and α-glucosidase) is one of many approaches used for the management of this disease. This study was aimed at evaluating the normoglycaemic potential of Helianthus annuus leaf. Methods: The effect o...

conclusions the current report isolates, characterizes, and demonstrates the novel heat-adapted amylase-producing bacteria ss1, ss2 and ss3 from taptapani hot spring, indicating its potentiality and stability under acidic conditions. materials and methods bacterial strains were isolated by dilution plating method from the water samples collected from taptapani hot spring, odisha and screened fo...