introduction: hela cell is a widely studied cell line derived from human cerzix adenocarcinoma. the cells are epithelial - like in morphology and susceptible to polio viruses and adenovirus type 5. hela cells are usually as monolayer for isolation and propagation of some human viruses, as well as for the expression of recombinant proteins, including hepatitis b surface antigen. the aim of this ...

background: our previous studies revealed an inhibitory effect of icd-85 (venom derived peptides) on mda-mb231 and hl-60 cell lines, through induction of apoptosis. the purpose of this study was to investigate apoptosis-induced mechanism on hela and mrc-5 cells by icd-85 through activation of caspase-8. methods: cell viability, cytosolic enzyme lactate dehydrogenase (ldh) and cell morphology we...

background: gemcitabine (2′, 2′-difluoro-2′- deoxycytidine, an analogue of deoxycytidine) is a relatively new drug with wide range of anti-cancer activity. in this study, radiosensitizing effects of gemcitabine was investigated on hela and mrc5 human originated cell lines under both chronically hypoxic and normoxic conditions using the micronucleus (mn) assay. materials and methods: for inducti...

based on the evaluation of two human cell lines, hela and chang, abeuploidy and several marker chromosomes were found in both cells. the morphological characteristic of marker chromosomes of chang cells was distinctly different from hela. certain submetacentric marker chromosome was frequently present among 80% of marker chromosomes of chang cells which distinguished this line from hela, which ...

background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

Background and purpose: Cytotoxic effects of Ligustrum vulgare leaves on HeLa cervical tumor cells suggested that extracts should be investigated as potential anticancer agents. Therefore, we examined a antileukemic activity methanolic fruit two types leukemia cells, MOLT-4 JVM-13, lymphocytes isolated from the blood 33 chronic lymphocytic (CLL) patients mononuclear leukocytes 18 healthy indivi...

Estradiol valerate (Exogenous estrogen) is a synthetic ester and is also a female estrogen hormone. There arestudies showing that estradiol may have anticancer effects. This study was performed to reveal the effects ofexogenous estrogens on the viability of cervical cancer cells in cell culture. We used Hela cells as our cell linein this study. Hela cells were exposed to 0.0001, 0.001, 0.01, 0....

The alkylating mutagens N-methyl-N'-nitro-N-nitrosoguanidine, methyl methanesulfonate, and N-nitroso-methylurea induced immunoreactivity to antinucleoside antibodies in human peripheral blood lymphocytes in vitro. This could also be detected in lymphocytes taken from a patient soon after i.v. administration of cyclophosphamide. The immunoreactivity response, which indicates denatured DNA or DNA...

Background: Artediffusin is a sesquiterpene lactone with an endoperoxide group which has been isolated from Artemisia diffusa. Artemisia has always been of great botanical and pharmaceutical interest and is useful in traditional medicines for the treatment of a variety of diseases and complaints. The aims of the present study were to evaluate the cytotoxic, mutagenic and anti-mutagenic activiti...

abstractbackgroundcontributes substantially to patient morbidity and mortality.in this study we investigatedthe range and distribution of t-lymphocyte. subsets cd3helper/inducer cell,.th ), cd8: severe immunosuppression occurs after large thermal burn and probably+ (t cells) cd4+ (t+ (t suppressor /cytotoxic cells ,ts/c), cd3+cd4thermal injury.+/cd3+cd8+ ratio, cd19+ (b cells) and cd16+ (nk cel...