Background and Aims: Hepatitis C is a blood-borne virus that causes a liver inflammation. The prevalence of hepatitis C (HCV) in hemodialysis patients is more than the general population. Periodic screening of HCV in dialysis centers is necessary to control this viral disease.  The aim of this study is the evaluation of HCV infection among hemodialysis patients living in Yazd province of...

Abstract: Genotyping of the hepatitis C virus (HCV) is important for designing therapeutic strategies and regional specific diagnostic assays. The aim of this study was to identify the HCV genotypes in HCV infected blood donors. This is the first report on HCV genotypes in blood donors in Iran. In this cross-sectional study, 103 blood donors with hepatitis C were investigated for HCV genotype...

Progress in understanding hepatitis C virus (HCV) biology has remained a challenge due to the lack of an efficient cell culture system for virus growth. In this study, we examined HCV core protein-mediated immortalized human hepatocytes (IHH) for growth of HCV. In vitro-transcribed full-length RNA from HCV genotype 1a (clone H77) was introduced into IHH by electroporation. Reverse transcription...

abstract: genotyping of the hepatitis c virus (hcv) is important for designing therapeutic strategies and regional specific diagnostic assays. the aim of this study was to identify the hcv genotypes in hcv infected blood donors. this is the first report on hcv genotypes in blood donors in iran. in this cross-sectional study, 103 blood donors with hepatitis c were investigated for hcv genotypes....

Hepatitis C virus (HCV), a positive-strand RNA virus, has been considered to have a poly(U) stretch at the 3' terminus of the genome. We previously found a novel 98-nucleotide sequence downstream from the poly(U) stretch on the HCV genome by primer extension analysis of the 5' end of the antigenomic-strand RNA in infected liver (T. Tanaka, N. Kato, M.-J. Cho, and K. Shimotohno, Biochem. Biophys...

Hepatitis C virus (HCV) preferentially replicates in the human liver and frequently causes chronic infection, often leading to cirrhosis and liver cancer. HCV is an enveloped virus classified in the genus Hepacivirus in the family Flaviviridae and has a single-stranded RNA genome of positive orientation. The HCV RNA genome is translated and replicated in the cytoplasm. Translation is controlled...

context hepatitis b and hepatitis c (hbv and hcv) infections are both major causes of hepatocellular carcinoma (hcc). however, hcc caused by each of these two viruses has unique characteristics that should be studied independently to that of another one. while hbv- and hcv-related hccs share similar host and environmental risk factors such as male gender, age above 50 years old, family history ...

background: hepatitis c is a global health problem. the exact mechanisms by which hepatitis c virus (hcv) can evade the host immune system have become controversial. whether hcv polyproteins modulate ifn signalling pathways or hcv proteins are responsible for such a property is the subject of interest. therefore, an efficient baculovirus delivery system was developed to introduce the whole geno...

22 23 Hepatitis C virus (HCV) reinfects liver allografts in transplant recipients, replicating 24 immediately after transplantation, followed by a rapid increase in serum HCV RNA 25 levels. We evaluated dynamic changes in the viral genetic complexity after HCV 26 reinfection of the graft liver and identified the characteristics of replicating HCV clones 27 using a massive-parallel ultra-deep se...

Qualitative detection of negative hepatitis C virus (HCV) RNA has been used widely to demonstrate HCV replication. However, relative quantitation of both positive and negative HCV RNA strands has never been reported for studying viral genome replication. A strand specific real-time PCR carried out in the highly conserved 5'-non-coding region of HCV genome and monitored either by the DNA binding...