Total glycosylated haemoglobin (HbA1) levels were measured in 94 diabetic children aged between 3 and 19 years. The results were compared with traditional methods of assessing blood glucose control. HbA1 levels correlated with home urine glucose testing (P less than 0.05), with 24-hour urine glucose excretion (P less than 0.01), and with height velocity (P less than 0.001). Within the first two...

1. Concentrations of high-density-lipoprotein cholesterol and of glycosylated haemoglobins [HbA1(a+b+c)] were measured in non-fasting blood samples taken from 171 diabetic patients. 2. Mean concentrations of high-density-lipoprotein cholesterol were lower in men, and in patients not requiring insulin. 3. There was no correlation between concentrations of high-density-lipoprotein cholesterol and...

The homology model of major haemoglobin component HbA1 of the African Clawed Frog was predicted using the pigeon (Columba livia) haemoglobin as a template. The model was built with the help of MODELLER9v8. The models were evaluated with ProSA and PROCHECK. In X. laevis Gln38α is unable to form a hydrogen bond with β97His or β99Asp, which is responsible for the increase in oxygen affinity of the...

Two Chinese patients with mild and moderate hemoglobin (Hb) H disease were investigated for rare mutations in the α-globin genes (HBA1, HBA2) in addition to --(SEA) deletion. One patient was a 41-year old man with mild anemia (Hb 11.3 g/dl). Multiplex ligation-dependent probe amplification (MLPA) revealed a rare 2392-base deletion involving the entire HBA1. Mapping by gap-polymerase chain react...

Total glycosylated haemoglobin (HbA1) levels from capillary blood were studied retrospectively during a 1-year period in 148 diabetic children aged between 1.9 and 16.8 years. The clinic range for HbA1 was 6.7 to 22.2% and the results were normally distributed (mean +/- SD 13.2 +/- 2.8%). The normal range in non-diabetics using this method is 4.9 to 8.0%. Results from children who had had diabe...

The human α-globin gene cluster is located on chromosome 16 pterp13.3, and is arranged in the order, 5’ζ2-ψζ1-ψα2-ψα1-α2-α1-θ1-3’. Recent reexamination of the ψα2 gene revealed that it is expressed at a very low level, and was renamed as mu-globin gene. The cluster was thought to result from duplication events that occurred more than 300 million years ago. Unequal homologous recombinations are ...

The apparent proportion of column-chromatographically measured glycosylated hemoglobin in erythrocytes from an individual with hemoglobins A, S, and G was only 2.3% because the slow glycosylated variant hemoglobins were retarded in the column. In contrast, the value for glycosylated hemoglobin was 7.8% by a new cellulose acetate electrophoretic method that includes use of dextran sulfate buffer...

Mutations in HBA1 and HBA2 genes cause α-thalassemia. Mutation in these two genes may produce deficient α subunit chains in hemoglobin in the red blood cells, and lead to anemia or even death. Since I was suspect of thalassemia, the objective of this study was to identify if there is any mutation in HBA1 and HBA2 genes in my genomic DNA. Using primers specific to the HBA1 and HBA2 genes, these ...

Total glycosylated haemoglobin (HbA1) levels from capillary blood were studied retrospectively during a 1-year period in 148 diabetic children aged between 1 9 and 16 8 years. The clinic range for HbA1 was 67 to 22 2% and the results were normally distributed (mean ± SD 13 2 ± 28 %). The normal range in non-diabetics using this method is 4 9 to 8-0 %. Results from children who had had diabetes ...

We studied the influence of many variables on the cation-exchange chromatographic determination of glycohemoglobin was minicolumns (HbA1) and with macrocolumns (HbA1a+b and HbA1c). Minicolumns are most sensitive to buffer composition. The best conditions are pH 6.78 and sodium, potassium, and phosphate concentrations of 54.8, and 36 mmol/L, respectively. In fact, there is no clear cut-off point...