Plasmids with both a mammalian replication initiation region (IR) and a matrix attachment region (MAR) are spontaneously amplified in transfected cells, and generate extrachromosomal double minute (DM) or chromosomal homogeneously staining region (HSR). We previously isolated the shortest core IR (G5) required for gene amplification. In this study, we ligated the G5 DNA to create direct or inve...

Sahel bölgesi, son on yıl içerisinde hem siyasi de ekonomik açıdan güvenlik sorununun belirginleştiği önemli bölgelerden birisi haline gelmiştir. bir yandan bölgesel ve bölge dışı güçlerin politikalarını etkileyen, diğer bu devletlerin terörizmle mücadele girişimlerini belirleyen coğrafya olarak ön plana çıkmıştır. Buna çalışmada, bölgesinde yaşanan terör saldırılarının nedenleri incelenmiş sal...

Here we show a correlation between telomere length and organismal sensitivity to ionizing radiation (IR) in mammals. In particular, fifth generation (G5) mouse telomerase RNA (mTR)(-/)- mice, with telomeres 40% shorter than in wild-type mice, are hypersensitive to cumulative doses of gamma rays. 60% of the irradiated G5 mTR(-/)- mice die of acute radiation toxicity in the gastrointestinal tract...

BACKGROUND The potential nanocarrier polyamidoamine (PAMAM) generation 5 (G5-NH(2)) dendrimer has been shown to evoke lasting neuronal depolarization and cell death in a concentration-dependent manner. In this study we explored the early progression of G5-NH(2) action in brain tissue on neuronal and astroglial cells. RESULTS In order to describe early mechanisms of G5-NH(2) dendrimer action i...

We investigate the dynamical evolution of the terrestrial planets during the planetesimal-driven migration of the giant planets. A basic assumption of this work is that giant planet migration occurred after the completion of terrestrial planet formation, such as in the models that link the former to the origin of the Late Heavy Bombardment. The divergent migration of Jupiter and Saturn causes t...

Gold nanoparticles (NPs) have recently received immense scientific and technological interest because of their extensive applications in biology, catalysis, and nanotechnology. In most of these applications, the gold NP surfaces are modified with different functionalities. For instance, alkanethiol and alkylamine molecules are used for phase transfer of gold NPs, and oligonucleotide molecules a...

Multiwalled carbon nanotubes (MWCNTs)amphiphilic poly(amidoamine) (PAMAM) dendrimers (APAMAM) based hybrids were developed through individual functionalization of PAMAM with generation 4 (G4) and generation 5 APAMAM (G5) on MWCNTs. The two hybrids were used as matrices and deposited with silver (Ag) nanoparticles (NPs) to develop another two types of new MWCNT based hybrids viz., MWCNTAPAMAM (G...

The effect of DPP grains, isolated flavonoids were carried out on the levels steroid hormone and Lipid profile in sera adult female rabbits.
 This animals divided into seven groups, which include. acontrol group, G1,G2,G3:groups orally treated with (12,24,36)mg/kg/day grain while groupsG4,G5,G6:orally (5,10,15) mg/kg/day DDP.
 -Testosterone significantaly increased G3,G4,G6 as compare...

The bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. In this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and these strains were assigned to 7 genera according to their 16S rDNA and they were Aeromonas, Bacill...