Isolates of widely distributed amoeba species Hartmannella vermiformis differs in the details of cyst structure. This is unusual for gymnamoebae and is important in course of modern approaches to amoebae systematic. Cysts of the isolate of this species, described here, have regular separation of the cyst wall into endocyst and ectocyst, in contrast with the “original” isolates, described by F.C...

background: poor hygiene will provide good condition for corneal infections by opportunistic free-living amoebae (fla) in soft contact lens wearers. in the present study an amoebic keratitis due to hartmannella has been recognized in a 22-year-old girl with a history of improper soft contact lens use. she had unilateral keratitis on her left eye. her clinical signs were eye pain, redness, blurr...

background: geothermal waters could be suitable niches for thermophilic free living amoebae includ­ing naegleria and hartmannella . ardebil province, northwest iran is popular for having many hot springs for recreational and health purposes activity. the present research is the first molecular based investigation regarding the presence of naegleria and hartmannella in the hot springs of ardebil...

Based on morphological and molecular characterisation, four amoeba strains isolated from organs of freshwater fish were identified as Hartmannella vermiformis Page, 1967. Small subunit rRNA gene sequences of these strains expand the set of corresponding complete and almost complete sequences of this species to twelve. A new species-specific oligonucleotide probe inferred from recently available...

Eighteen cases of Acanthamoeba-associated keratitis among contact lens wearers seen at the Department of Ophthalmology, Karl-Franzens-University, Graz, Austria, between 1996 and 1999 are reviewed. The amoebae were proven to be the causative agents in three patients. The aim of our study was to discriminate between clinically relevant and nonrelevant isolates and to assess the relatedness of the...

The aim of this study was to determine the presence of free-living amebae in aquatic habitats of human environments in Thailand and Hamamatsu district, Japan. Genus identification was based on the morphology of cyst and trophozoite forms and a flagellation test for genus Naegleria. The pathogenic potential was tested in mice by nasal instillation for genus Naegleria and Acanthameba. In 14 provi...

Overwhelming contamination of a continuous cell line with a free-living soil amoeba of the genus Hartmannella is reported. The significance of the finding is discussed because of recent interest shown in the role of this and related species of amoebae in disease of humans. The methods used to detect and identify the amoeba are described and some examples of the amoebae are shown by photomicrogr...