Lipolysis is the catabolic pathway by which triglycerides are hydrolyzed into fatty acids. Adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) have the capacity to hydrolyze in vitro the first ester bond of triglycerides, but their respective contributions to whole cell lipolysis in human adipocytes is unclear. Here, we have investigated the roles of HSL, ATGL, and its coactiv...

Adipocytes isolated from abdominal fat of female broilers and maintained in primary culture were used to characterize acute and chronic effects of somatostatin (SRIF) on lipolysis and antilipolysis and to determine whether desensitization and cross-regulation phenomena are involved in the regulation of adipocyte metabolism by SRIF and glucagon. Acute exposure of adipocytes to SRIF resulted in a...

During the fasting state, insulin reduces nonesterified fatty acid (NEFA) appearance in the systemic circulation mostly by suppressing intracellular lipolysis in the adipose tissue. In the postprandial state, insulin may also control NEFA appearance through enhanced trapping into the adipose tissue of NEFA derived from intravascular triglyceride lipolysis. To determine the contribution of suppr...

The prediction of the in vivo performance self-nanoemulsifying drug delivery systems (SNEDDSs) is currently gaining increasing attention. Therefore, need for reliable vitro models able to assess solubilization capacity such formulations upon lipolysis, as well concomitantly evaluate permeation, has become ever so evident. In current study, high-throughput intestinal lipolysis model was combined...

This study assessed the effects of selective inhibitors of 3',5'-cyclic nucleotide phosphodiesterases (PDEs) on adipocyte lipolysis. IC224, a selective inhibitor of type 1 phosphodiesterase (PDE1), suppressed lipolysis in murine 3T3-L1 adipocytes (69.6 +/- 5.4% of vehicle control) but had no effect in human adipocytes. IC933, a selective inhibitor of PDE2, had no effect on lipolysis in either c...

OBJECTIVE Fatty acids released via fat cell lipolysis can affect circulating lipid levels. However, the contribution of different lipolysis measures in adipose tissue is unknown and was presently examined in isolated subcutaneous adipocytes. APPROACH AND RESULTS One thousand and sixty-six men and women were examined for lipolysis regulation in subcutaneous abdominal fat cells. Results were co...

Successful adaptation to starvation in mammals depends heavily on the regulated mobilization of fatty acids from triacylglycerols stored in adipose tissue. Although it has long been recognized that cyclic AMP represents the critical second messenger and hormone-sensitive lipase (HSL)**Abbreviations used in this paper: ADRP, adipocyte differentiation-related protein; HSL, hormone-sensitive lipas...

The aim of this work was to develop a new in vitro lipolysis-permeation model predict the vivo absorption fenofibrate self-nanoemulsifying drug delivery systems (SNEDDSs). More specifically, intestinal lipolysis combined with mucus-PVPA (Phospholipid Vesicle-based Permeation Assay) permeability model. Biosimilar mucus (BM) added surface PVPA barriers closer simulate mucosa. SNEDDSs for which ph...

(Log dose)-response curves have been determined for lipolysis and for the conversion of glucose-(14)C to (14)CO(2) by adipose tissue from rats in the presence of epinephrine, corticotropin, and thyrotropin. The stimulatory effect of epinephrine on lipolysis was greater than that of corticotropin or thyrotropin. Lipolysis induced by epinephrine was inhibited by propranolol but only slightly by p...

Agonist-induced lipolysis of adipose fat is robustly inhibited by insulin or by feedback inhibition by the long-chain fatty acids (LCFA) produced during lipolysis. However, the mode of action of LCFA in suppressing adipose lipolysis is not clear. β,β'-Tetramethyl hexadecanedioic acid (Mββ/ EDICA16) is a synthetic LCFA that is neither esterified into lipids nor β-oxidized, and therefore, it was ...