agrobacterium rhizogenes causes hairy root disease in plants. these hairy roots are ge-netically stable and grow rapidly. transformed hairy roots of hyoscyamus muticus in-duced by the bacterium can produce tropane alkaloids in trace amounts of intact plant tissues. in this research, in order to compare growth and biosynthetic stability of hairy roots with wild type ones, leaf and nodal segments...

Linum spp. (Linaceae) has been shown to accumulate cytotoxic lignans such as podophyllotoxin and 6-methoxypodopyllotoxin. Therefore, plant cell and tissue cultures of Linum spp. may be possible alternative sources for the lignan production. In this study, we focused on the production of lignans by root cultures of Linum spp. Hairy root cultures of L. nodiflorum, L.album and L. austriacum and ro...

A method named "bag culture" was developed for coculturing of Linum persicum (section Syllinum) and L. austriacum (section Linum) hairy roots. For this propose L. austriacum and L. persicum hairy root cultures were established using Agrobacterium rhizogenes in McCown medium. L. persicum hairy roots in bags (1 mm2 mesh) were successfully grown together with L. austriacum hairy roots. The amounts...

silymarin production by hairy root culture of milk thistle (silybum marianum l. gaertn) was investigated using agrobacterium rhizogenes ar15834. hairy roots were induced by injection or inoculation of explants with a. rhizogenes. one month old hairy roots were dissected from the explants and grown in murashing and skoog (ms) liquid medium. polymerase chain reaction (pcr) using the b gene and th...

Silymarin production by hairy root culture of Milk thistle (Silybum marianum L. Gaertn) was investigated using Agrobacterium rhizogenes AR15834. Hairy roots were induced by injection or inoculation of explants with A. rhizogenes. One month old hairy roots were dissected from the explants and grown in Murashing and Skoog (MS) liquid medium. Polymerase Chain Reaction (PCR) using the B gene and th...

Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to imp...

Hairy root culture is a potential alternative to conventional mammalian cell culture to produce recombinant proteins due to its ease in protein recovery, low costs and absence of potentially human pathogenic contaminants. The current study focussed to develop a new platform of a hairy root culture system from Nicotiana tabacum for the production of recombinant human EPO (rhEPO), which is regula...

Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...

Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...