The influence of microclimate on numbers of third-state larvae of haemonchus contortus and Haemonchus placei in four strata of irrigated Kikuyu pasture was assessed. On 36 different, interspersed days three replicates of pasture samples were collected on three occasions per day from 1990-1992 for larval recovery and the log10 mean counts of the larvae recovered were analysed by use of ANOVA mod...

background: to use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. methods: peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of hae­monchus contortus were determined using sds-page and western blotting. five lambs were infected with 10,000 third stage larv...

ABSTRAK Kiraan telur cadng dalam najis dan kehadiran spesis-spesis nematod pada kambing daripada tujuh kandang kambing yang kedl di daerah Timur Laut, Pulau Pinang telah dikaji. Secara amnya kiraan telur adalah tinggi, berjulat antara 500 2000 epg. Keadaan iklim didaerah ini adalah sesuai bagi kehidupan dan perkembangan sekurang-kurangnya lima spesis nematod iaitu Cooperia spp., Haemonchus spp....

Molting is required for progression between larval stages in the life cycle of nematodes. We have identified four mutant alleles of a Caenorhabditis elegans metalloprotease gene, nas-37, that cause incomplete ecdysis. At each molt the cuticle fails to open sufficiently at the anterior end and the partially shed cuticle is dragged behind the animal. The gene is expressed in hypodermal cells 4 ho...

Haemonchus populations were collected from cattle from mid-western and eastern Southern US (four and six populations, respectively) to determine the relative prevalence of Haemonchus contortus and Haemonchus placei and the frequency of the three isotype-1 β-tubulin polymorphisms associated with benzimidazole resistance. A minimum of 32 individual adult worms were genotyped at position 24 of the...

Debates continue over the extent to which the parasitic trichostrongylids Haemonchus placei and Haemonchus contortus hybridise in nature, and whether they deserve species status. Mitochondrial ND4 gene sequences from individuals of each putative species collected from populations around the United States indicate that the two species are highly differentiated at the mtDNA level. Furthermore, th...

Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

Fifty-thousand infective larvae of Trichostrongylus axei dosed to 7-9-month-old Merinos protected them against a subsequent challenge with 50 000 infective larvae of Haemonchus contortus. Protection varied with the period between an initial dosage of T. axei and challenge with H. contortus as follows: After 90 days it was more than 80% effective in more than 80% of sheep; at 119 and 180 days th...

A field program to control multiple anthelmintic resistant worm infections in cattle of Argentina is described. In 2003 around 140 calves died as a consequence of high worm burdens despite the administration of monthly alternate treatments with ivermectin (IVM) or fenbendazole (FBZ). A fecal egg count reduction test (FECRT) showed a clinical efficacy of 73%, 49.1% and 90.4% for IVM, FBZ and lev...