Sera from various wild ruminants co-existing in the same habitats with cattle had haemagglutination-inhibition (HI) antibodies as high as those in the cattle. Sera from wildebeest (Connochaetes taurinus), kongoni (Alcelaphus cokei), Thomson’s gazelle (Gazella thomsonii), eland (Taurotragus oryx) and the African buffalo (Syncerus caffer) had titers ranging from 64 to 128. Zebra (Equus burchelli)...

An examination of 200 serum samples from unvaccinated indigenous (local) chickens in Maiduguri, Borno State (Nigeria) using the haemagglutination inhibition (HI) test showed 73 sera to be positive and 127 to be negative for antibodies against Newcastle disease virus. The highest antibody titre observed was 1:128. The prevalence rate was higher (46.9%) in adult chickens than in young chickens of...

PathWest Laboratory Medicine WA detected RRV IgM antibodies using an inhouse indirect immunofluorescence antibody (IFA) test, but no RRV antibodies were detected using an inhouse haemagglutination inhibition (HI) antibody test 10 days after blood donation. RRV IgM antibodies are detected by IFA testing within a few days of onset of illness and routinely persist for several weeks or, occasionall...

Sera obtained from 777 pigs were tested for the presence of Sendai virusimmunospecific antibodies by the agar gel immunodiffussion (AGID), counterimmunoelectrophoresis (CIE), haemagglutination inhibition (HI) and serum neutralisation (SN) tests. As many as 598 sera contained Sendai virus antibodies as shown by the SN test, 552 sera were HI positive, 536 sera were CIE positive, and only 444 ones...

A rapid whole cell protein antigen (iron restricted Mycoplasma gallisepticum) based latex agglutination test (LAT) was developed to detect the presence of antiMycoplasma gallisepticum antibodies in the sera of poultry affected with mycoplasmosis. The test was found to be sensitive, specific and accurate in comparison with Haemagglutination Inhibition (HI) test. The rapidity, simplicity and econ...

Newcastle disease is one of the most important diseases of poultry. It usually causes a great loss inpoultry industry and domestic village chickens. Since domestic chickens in villages are free ranging for food,the chance of their contact with wild birds that may act as reservoirs is high. To determine the role ofdomestic chickens in the epizootiology of Newcastle disease virus in villages of I...

The aim of this study was to investigate the prevalence of CIV H3N8 in dogs in Germany. Blood samples from 272 clinically healthy dogs and 35 dogs with acute respiratory signs were screened for antibodies against influenza virus by ELISA and haemagglutination inhibition (HI) assay; positive samples were further tested by fluorescent antibody test (FAT) and subtype-specific HI assay. Nasal and p...

Sera from 1000 women attending antenatal clinics were screened for the presence of rubella antibody by haemagglutination inhibition (HI), single radial haemolysis (SRH), and enzyme-linked immunosorbent assay (ELISA). With 925 sera concordant results were obtained with all three tests. There were 46 false-negative HI tests due to the necessity of allowing for residual non-specific inhibitors. Wi...

this experiment was conducted for evaluating the humoral immune responses induced by poly lactide-co-glycolide acid (plga) microspheres coupled inactivated newcastle disease virus (ndv) vaccine in comparison to an ‘in-house’ prepared inactivated and a live commercial vaccine. plg microparticles containing inactivated ndv were prepared by a double emulsion technique based on solvent evaporation ...

PAWESKA, J.T. , BARNARD, B.J.H. & WILLIAMS, R. 1995. The use of sucrose-acetone-extracted Rift Valley fever virus antigen derived from cell culture in an indirect enzyme-linked immunosorbent assay and haemagglutination-inhibition test. Onderstepoort Journal of Veterinary Research, 62:227-233 A sucrose-acetone-extracted , Madin-Darby-bovine-kidney (MDBK)-derived Rift Valley fever virus (RVFV) an...