in this study, the pathogenicity of a/ch/it/5093/1999 h7n1 which had been isolated from chicken during the outbreak in italy was assessed in chicken by experimental infection virus. ten spf chickens of four week-old were inoculated with this virus, and five chickens were inoculated with uninfected allantoic fluid. for determination of virus shedding, oropharyngeal and cloacal swabs were taken f...

Large-scale sequence analyses of influenza viruses revealed that nonstructural 1 (NS1) proteins from avian influenza viruses have a conserved C-terminal ESEV amino acid motif, while NS1 proteins from typical human influenza viruses have a C-terminal RSKV motif. To test the influence of the C-terminal domains of NS1 on the virulence of an avian influenza virus, we generated a wild-type H7N1 viru...

We report the genome sequence of an avian influenza virus (AIV) subtype H8N8, isolated in Russia. The genome analysis shows that all genes belong to AIV Eurasian lineages. The PB2 gene was similar to a Mongolian low-pathogenic (LP) AIV H7N1 and a Chinese high-pathogenic (HP) AIV H5N2.

Zoonotic influenza H7 viral infections have a case fatality rate of about 40%. Currently, no or limited human to human spread has occurred, but we may be facing a severe pandemic threat if the virus acquires the ability to transmit between humans. Novel vaccines that can be rapidly produced for global distribution are urgently needed, and DNA vaccines may be the only type of vaccine that allows...

Inappropriately named gain-of-function influenza research seeks to confer airborne transmission on avian influenza A viruses that otherwise cause only dead-end infections in humans. A recent study has succeeded in doing this with a highly pathogenic ostrich H7N1 virus in a ferret model without loss of virulence. If transposable to humans, this would constitute a novel virus with a case fatality...

Introduction Highly pathogenic avian influenza (HPAI) is a markedly contagious viral disease of domestic chickens and turkeys, characterized by severe illness with high morbidity and mortality (Easterday et al. 1997). Human infections with the HPAI virus H5N1 frequently cause death since 1997 (Class et al., 1998, Subbarao et al., 1998). Two surface glycoproteins, the haemagglutinin (HA) and neu...

In this study, the pathogenicity of A/Ch/It/5093/1999 H7N1 which had been isolated from chicken during the outbreak in Italy was assessed in chicken by experimental infection virus. Ten SPF chickens of four week-old were inoculated with this virus, and five chickens were inoculated with uninfected allantoic fluid. For determination of virus shedding, oropharyngeal and cloacal swabs were taken f...

following experimental inoculation of 3-week-old turkeys with different titres (106, 104, 103, 102 and 101 egg infectious dose (eid50)) of a/ostrich/italy/984/2000 h7n1 highly pathogenic avian influenza virus (hpaiv), the selected tissues and organs were examined for pathological changes. tissue samples from different organs that obtained from dead and sacrificed birds were fixed in 10% neutral...