in this study the role of glutathione (gsh) in sulfur mustard -induced oxidative stress and cytotoxicity, in human skin fibroblast cell line (hf2ff) was evaluated. sulfur mustard-induced superoxide radical and hydrogen peroxide formation were evaluated by determination of superoxide dismutase and catalase activity in cell lysate. the cytotoxicity of sulfur mustard was estimated by lactate dehyd...

Regulation of the cysteine transporter known as excitatory amino acid carrier-1 (EAAC1) for intracellular glutathione (GSH) content was investigated using human embryonic kidney (HEK) 293 cells as a model system. GSH content was significantly reduced by l-aspartate-beta-hydroxamate (50-250 microM), an inhibitor of both EAAC1 and GLT1, both of which are transporters to take up cysteine, whereas ...

Four pig hybrids (Pannon, Hungahib-39, Középtiszai and Dalland) were fattened up to 100±2 kg body weight. Feed intake and body weight were measured and daily weight gain was calculated. Malondialdehyde (MDA) and reduced glutathione (GSH) content, and glutathione peroxidase (GPx) activity were measured in liver and kidney. Daily weight gain was significantly lower in Pannon and Hungahib-39 hybri...

The study was designed to investigate the role of magnesium (Mg) pretreatment on reduced glutathione (GSH) levels in kidney, liver and testis of mice intoxicated with cadmium (Cd). Animals were divided into four groups: I--controls, II--Cd group: mice intoxicated with Cd, III--Mg+Cd group: mice given Mg 1 h before Cd, and IV--Mg group: mice given only Mg. In acute time--response studies, the si...

Intracellular glutathione (GSH) has been shown to be one of the major factors modulating tumor response to a variety of commonly used anti-neoplastic agents. In this study the GSH contents of human ovarian tumors from primary biopsies, nude mouse xenografts, and in vitro cell cultures were compared. Pronounced intratumor cell-to-cell heterogeneity in GSH content was observed in primary patient ...

Liu, Rui-Ming, Yanwen Liu, Henry Jay Forman, Mitchell Olman, and Margaret M. Tarpey. Glutathione regulates transforming growth factor-stimulated collagen production in fibroblasts. Am J Physiol Lung Cell Mol Physiol 286: L121–L128, 2004. First published September 5, 2003; 10.1152/ajplung.00231.2003.—Transforming growth factor(TGF) is a potent fibrogenic cytokine. The molecular mechanism underly...

B16 melanoma (B16M) cells with high glutathione (GSH) content show rapid proliferation in vitro and high metastatic activity in the liver in vivo. gamma-Glutamyl transpeptidase (GGT)-mediated extracellular GSH cleavage and intracellular GSH synthesis were studied in vitro in B16M cells with high (F10) and low (F1) metastatic potential. GGT activity was modified by transfection with the human GG...

Glutathione (GSH) concentration increases in bovine oocytes during in vitro maturation (IVM). The constitutive amino acids involved in GSH synthesis are glycine (Gly), glutamate (Glu) and cysteine (Cys). The present study was conducted to investigate the effect of the availability of glucose, Cys, Gly and Glu on GSH synthesis during IVM. The effect of the amino acid serine (Ser) on intracellula...

Here we investigate the mechanism(s) involved in the c-Myc-dependent drug response of melanoma cells. By using three M14-derived c-Myc low-expressing clones, we demonstrate that alkylating agents, cisplatin and melphalan, trigger apoptosis in the c-Myc antisense transfectants, but not in the parental line. On the contrary, topoisomerase inhibitors, adriamycin and camptothecin, induce apoptosis ...

Transforming growth factor-beta (TGF-beta) is a potent fibrogenic cytokine. The molecular mechanism underlying TGF-beta fibrogenesis, however, has not been completely elucidated. In this study, we showed that TGF beta decreased the intracellular GSH content in murine embryo fibroblasts (NIH 3T3), which was followed by an increase in collagen I mRNA content and collagen protein production. Preve...