in this paper we investigate the green‎ ‎graphs for the regular and inverse semigroups by considering the‎ ‎green classes of them‎. ‎and by using the properties of these‎ ‎semigroups‎, ‎we prove that all of the five green graphs for the‎ ‎inverse semigroups are isomorphic complete graphs‎, ‎while this‎ ‎doesn't hold for the regular semigroups‎. ‎in other words‎, ‎we prove‎ ‎that in a regular se...

Real-time RT-PCR and SYBR green I melt curve analysis of a 74 bp amplicon enabled identification of Plum pox virus strains C, EA, and W, with distinct T(m)'s associated with each strain. This test is a useful supplement to a real-time RT-PCR test described earlier that was used to distinguish PPV strains D and M. A longer fragment of 155 bp was not effective for strain identification. A simplif...

Following the initial report of the use of SYBR Green I for real-time polymerase chain reaction (PCR) in 1997, little attention has been given to the development of alternative intercalating dyes for this application. This is surprising considering the reported limitations of SYBR Green I, which include limited dye stability, dye-dependent PCR inhibition, and selective detection of amplicons du...

chlorophyll fluorescence induction is a rapid and noninvasive technique for measuring photosynthetic electron transport in plants. chlorophyll fluorescence measurements were performed under greenhouse condition to characterize how the fluorescence induction curves (kautsky curve) and its parameters were affected by metribuzin in susceptible and resistant junglerice (echinochloa colona (l.) link...

background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...

Some twelve years ago, Mark Green [G] made a few conjectures regarding the behaviour of syzygies of a curve C imbedded in IP n by a complete linear system. The so-called generic Green conjecture on canonical curves pertains to this question when the linear system is the canonical one and the curve is generic in the moduli, and predicts what are the numbers of syzygies in that case. Green and La...