Granulopoiesis is tightly regulated to meet host demands during both " steady-state " and " emergency " situations, such as infections. The transcription factor CCAAT/enhancer binding protein b (C/EBPb) plays critical roles in emergency granulopoiesis, but the precise developmental stages in which C/EBPb is required are unknown. In this study, a novel flow cytometric method was developed that s...

Infections and inflammation trigger neutrophilias that are supported by a hematopoietic program of accelerated granulopoiesis known as emergency granulopoiesis. The intrinsic factors that drive reactive neutrophilias and emergency granulopoiesis have been inferred but not demonstrated. Here, we show that alum cannot elicit reactive neutrophilias in IL-1R type I (IL-1RI)(-/-) mice, whereas other...

Acute promyelocytic leukemia (APL) is always associated with chromosomal translocations that disrupt the retinoic acid receptor alpha (RARalpha) gene. Whether these translocations relate to a role for endogenous RARalpha in normal granulopoiesis remains uncertain because most studies addressing this question have used non-physiological overexpression systems. Granulocyte differentiation in cell...

Abstract Emergency granulopoiesis, also known as demand-adapted is defined the response of an organism to systemic bacterial infections, and it results in neutrophil mobilization from reservoir pools increased myelopoiesis bone marrow. Indirect direct initiating mechanisms emergency granulopoiesis have been hypothesized. However, detailed mechanism hyperactive marrow, which leads granulocyte le...

Emergency granulopoiesis is a component of the innate immune response that is induced in response to infectious or inflammatory challenge. It is characterized by the rapid expansion and differentiation of granulocyte/monocyte progenitor (GMP) populations, which is due in part to a shortened S-phase of the cell cycle. We found that IRF8 (also known as ICSBP), an interferon regulatory transcripti...

Granulocyte colony-stimulating factor (G-CSF) mediates “emergency” granulopoiesis during infection, a process that is mimicked by clinical G-CSF use, yet we understand little about the intracellular signaling cascades that control demand-driven neutrophil production. Using a murine model with conditional deletion of signal transducer and activator of transcription 3 (STAT3) in bone marrow, we i...

Granulocyte colony-stimulating factor (G-CSF) mediates "emergency" granulopoiesis during infection, a process that is mimicked by clinical G-CSF use, yet we understand little about the intracellular signaling cascades that control demand-driven neutrophil production. Using a murine model with conditional deletion of signal transducer and activator of transcription 3 (STAT3) in bone marrow, we i...

Acute promyelocytic leukemia (APL) is always associated with chromosomal translocations that disrupt the retinoic acid receptor a (RARa) gene. Whether these translocations relate to a role for endogenous RARa in normal granulopoiesis remains uncertain because most studies addressing this question have used nonphysiological overexpression systems. Granulocyte differentiation in cells derived fro...

In this paper the modeling of the human granulopoiesis is carried out. This modeling is an important part of the further development of a decision support system to assist medical doctors in the treatment of irradiation injuries. Referring to an existing model of Fliedner and Steinbach we derive a new nonlinear time delay model of the human granulopoiesis with state dependent delays. Simulation...