Arenaviruses cause severe human disease ranging from aseptic meningitis following lymphocytic choriomeningitis virus (LCMV) infection to hemorrhagic fever syndromes following infection with Guanarito virus (GTOV), Junin virus (JUNV), Lassa virus (LASV), Machupo virus (MACV), Sabia virus (SABV), or Whitewater Arroyo virus (WWAV). Cellular immunity, chiefly the CD8(+) T-cell response, plays a cri...

High levels of ether phospholipids were found in rat platelets. Alkylacyl compounds constituted 18 and 29% of glycerophosphocholine (GPC) and glycerophosphoethanolamine (GPE). Alkenylacyl compounds, not detected in GPC, represented 40% of GPE. Arachidonate comprised 60%, 42% and 26% of the acyl residues in the sn-2 position of alkenylacyl-GPE, alkylacyl-GPE and alkylacyl-GPC respectively. Based...

High grain protein content (GPC) is a frequent target of wheat breeding programmes because of its positive effect on bread and pasta quality. A wild wheat allele at the Gpc-B1 locus with a significant impact on this trait was identified previously. The precise mapping of several senescence-related traits in a set of tetraploid recombinant substitution lines (RSLs) segregating for Gpc-B1 is repo...

Junin virus (JUNV), a highly pathogenic New World arenavirus, is the causative agent of Argentine hemorrhagic fever (AHF). The live-attenuated Candid #1 (Can) strain currently serves as a vaccine for at-risk populations. We have previously shown that the Can glycoprotein (GPC) gene is the primary gene responsible for attenuation in a guinea pig model of AHF. However, the mechanisms through whic...

In this paper we focus on the long-term behavior of generalized polynomial chaos (gPC) and multi-element generalized polynomial chaos (ME-gPC) for partial differential equations with stochastic coefficients. First, we consider the one-dimensional advection equation with a uniform random transport velocity and derive error estimates for gPC and ME-gPC discretizations. Subsequently, we extend the...

Expectation Propagation (EP) is an approximate Bayesian inference technique which has been applied to Gaussian Process Classification (GPC) [4]. In this paper, we investigate four different likelihood functions of GPC, and present EP algorithms for each of these four models. We compare the performances of these models on synthetic data in circular shape. Comparative study is performed on EP-GPC...

Light shaping techniques based on phase-only modulation o er multiple advantages over amplitude modulation. This review examines and compares the merits of two phase modulation techniques; phase-only computer generated holography and Generalized Phase Contrast (GPC). Both techniques are brie y presented while recent developments in GPC will also be covered. Furthermore, novel hybrid schemes tha...

BACKGROUND Glypican 3 (GPC-3) is an oncofoetal protein that is expressed in most hepatocellular carcinomas (HCC). Since it is a potential target for T cell immunotherapy, we investigated the generation of functional, GPC-3 specific T cells from peripheral blood mononuclear cells (PBMC). METHODS Dendritic cells (DC) were derived from adherent PBMC cultured at 37 degrees C for 7 days in X-Vivo,...

A vasodepressor phospholipid fraction named Depressor-IA from a bovine brain lipid extract was analyzed by capillary gas-liquid chromatography-mass spectrometry as tert-butyldimethylsilyl derivatives after hydrolysis with phospholipase C. Results show that Depressor-IA is a mixture of 3 platelet-activating factors and 17 1-acyl analogues. Three platelet-activating factors having sn-1-0-hexadecy...

Generation of infectious arenavirus-like particles requires the virus RING finger Z protein and surface glycoprotein precursor (GPC) and the correct processing of GPC into GP1, GP2, and a stable signal peptide (SSP). Z is the driving force of arenavirus budding, whereas the GP complex (GPc), consisting of hetero-oligomers of SSP, GP1, and GP2, forms the viral envelope spikes that mediate recept...