Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

Glycosylphosphatidylinositols (GPIs) are ubiquitous glycolipids in eukaryotes. In the protozoan Leishmania major, GPIs occur "free" or covalently linked to proteins (e.g., gp63) and polysaccharides. While some free GPIs are detected on the plasma membrane, specific sites where GPIs accumulate intracellularly are unknown in most cells, although the glycolipids are synthesized within the secretor...

background : production of monoclonal antibodies to leishmania antigens assists the identification and characterization of these organisms. objective: production of monoclonal antibodies against epitopes on the gp63. methods: two murine monoclonal antibodies to gp63 were produced and characterized. the reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

GP63 or leishmanolysin is the major surface protease of Leishmania spp. involved in parasite virulence and host cell interaction. As such, GP63 is a potential target of eventual vaccines against these protozoa. In the current study we evaluate the polymorphism of gp63 in Leishmania (Viannia) braziliensis isolated from two sets of American tegumentary leishmaniasis (ATL) cases from Corte de Pedr...

Acid proteinase activity is associated with the major surface glycoprotein (gp63) of both extracellular promastigotes and intracellular amastigotes of the parasitic protozoan, Leishmania mexicana. The enzyme purified by monoclonal affinity chromatography from promastigotes is strongly inhibited by metal ion chelators, which is reversible by the addition of Zn(II). This proteinase loses its acti...

Cysteine peptidases play a central role in the biology of Leishmania. In this work, we sought to further elucidate the mechanism(s) by which the cysteine peptidase CPB contributes to L. mexicana virulence and whether CPB participates in the formation of large communal parasitophorous vacuoles induced by these parasites. We initially examined the impact of L. mexicana infection on the traffickin...

Plant and insect trypanosomatids constitute the "lower trypanosomatids", which have been used routinely as laboratory models for biochemical and molecular studies because they are easily cultured under axenic conditions, and they contain homologues of virulence factors from the classic human trypanosomatid pathogens. Among the molecular factors that contribute to Leishmania spp. virulence and p...

Leishmanolysin (EC 3.4.24.36) (gp63) is a zinc metalloprotease (ND Rawlings et al. 1995 Methods Enzymol 248: 183-228) abundantly expressed in the promastigote form of Leishmania parasites, where it is attached to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor (P Schneider et al. It has been suggested that gp63 becomes active at multiple steps during the process of macrophage...

Background: Leishmania is an intracellular protozoan parasite that uses complex methods for destroying the innate immune response in mammalian host macrophage cells. Many factors have been identified play a role severity of parasite’s pathogenicity. One GP63, which group metalloproteinases disrupts signaling mechanism cell. Objectives: The aim this study was to construct PX-LMGP63 vector throug...