the development of culture media able to mimic the preovulatory stage of follicular environment and support nuclear and cytoplasmic maturation of oocyte is important for in-vitro fertilization (ivf) programs. it seems that the best culture media for embryonic development and cell cleavage is the natural composition which surrounds the oocyte which has been used occasionally in human ivf program...

background: fatty acids are known to be critically important in multiple biological functions. phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women’s fertility and the efficacy of assisted reproduction techniques. the aim of this study was to investigate the effect of fatty acid composition of follicular fluid ph...

background: it is reasonable to think that some biochemical characteristics of follicular fluid (ff) surrounding the oocyte may play a critical role in determining the quality of oocyte and the subsequent potential needed to achieve fertilization and embryo development. objective: this study was carried out to evaluate the levels of ff homocysteine (hcy) in ivf candidate polycystic ovary syndro...

Background: To evaluate whether success of fertilization and embryo quality are more probable when follicular fluid Anti-mullerian hormone levels are higher . Materials and Methods: 62 women who underwent IVF cycle were divided into fertilized oocytes group (n=42) and non- fertilized oocytes group (n=20) . Follicular fluid Anti-mullerian hormone levels were measured in both groups. Results: Ant...

background: the relationship between metabolism and reproduction has been always considered as an important topic in female endocrinology. it seems that leptin is one of the involved factors in infertility. leptin, in addition to regulating body weight plays an important role in regulation of endocrine, reproductive and immune systems. the aim of this stduy is to compare serum and follicular fl...

to study changes in concentrations of some biochemical factors of amniotic fluid, 115 normal goat uteri at different stages of pregnancy were collected from slaughterhouse. after expelling of each fetus accompanied by fetal membranes, 10 ml amniotic fluid was taken from amniotic sac for biochemical analysis. then approximate ages of fetuses were calculated by using age estimation formula. at fi...

Background: Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women’s fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid ph...

The aims of this study were to verify the steady-state level of vasoactive intestinal peptide (VIP) mRNA in goat follicles at various developmental stages and to investigate the influence of VIP on the survival, antrum formation and growth of secondary follicles cultured for 6 days. Primordial, primary and secondary goat follicles and small and large antral follicles were obtained to quantify V...

The ovarian follicular fluid was found to contain steroidogenesis stimulatory protein similar to albumin from human and buffalo. Therefore, the albumins from various species, commercial and purified, were studied for their steroidogenic effect on progesterone secretion by granulosa cells from buffalo ovaries, during culture. A dose of 20 μg of bovine serum albumin was optimum to exhibit maximum...