The levels of the important plant growth regulator indole-3-acetic acid (IAA) are tightly controlled within tissues to spatiotemporally orchestrate concentration gradients that drive and development. Metabolic inactivation bioactive IAA is known participate in modulation maxima minima. can be irreversibly inactivated by oxidation conjugation aspartate glutamate. Usually overlooked because its r...

Glycosyltransferases (GTs) catalyze the formation of glycosidic bonds in carbohydrates and glycoconjugates, with various outcomes depending not only on acceptor molecules they bind but also type bond form (C–O, C–N, C–S, or C–C). Here we show that glucosyltransferase UGT1 from indigo plant Polygonum tinctorium catalyzes either N-, O-, S-glycosylation similar rates. We solve structure enzyme com...

the culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mabs) production. the quality by design (qbd) approach defines the assured quality of the final product through the development stage. an important step in qbd is determination of the main quality attributes. during the media optimization, some of the main quality attribute...

Background: Diabetes is a metabolic disorder characterized by hyperglycemia due to the body’s inability produce insulin or inaction of produced combination both. One antidiabetic therapeutic approach reduce gastrointestinal glucose production and absorption through inhibition carbohydrate digesting enzymes such as alpha-amylase well hemoglobin glycosylation.
 Objective: This study sets out...

Lipopolysaccharides from Bacteroides vulgatus represent interesting targets for the treatment of inflammatory bowel diseases. In their Research Article (e202301351), Guozhi Xiao and co-workers accomplished first synthesis a tridecasaccharide motif lipopolysaccharides by strategy involving orthogonal one-pot glycosylation reactions based on glycosyl ortho-(1-phenylvinyl)benzoates, which preclude...

Glycoproteins are involved in various significant biological processes and have critical functions physiology pathology by regulating activities molecular signaling pathways. The variety of enzymes used protein glycosylation the wide range diversity resulting glycoproteins pose a challenging task when attempting to simulate these silico. This study aimed establish define necessary structures pr...

A disposable pump-free electrochemical microfluidic device made by using a multilayer lamination technique is proposed for diagnosis of glycosylation disorders. Thanks to the stacking polyethylene terephthalate (PET) films and double adhesive layers, it possible create geometry that allows filling tailored channels in fixed time. In main channel this passive device, necessary steps perform dete...

Background: Allergens are mostly composed of glycoprotein structures. It is believed that glycan-specific antibodies may lead to false-positive reactions in immunoassays. In this study we investigated the glycosylation state of grape allergens as well as the presence of antibodies to cross-reactive carbohydrate determinants (anti-CCDs) in sera from grape-sensitive individuals. Methods: Grape...

Abstract In this paper, we studied the inhibitory effect of oleanolic acid (OA) on non-enzymatic glycosylation and improvement glycometabolism in insulin resistant (IR) human liver tumour (HepG2) cells. The anti-glycosylation activity OA was determined by bovine serum albumin (BSA) fructose model. results showed that moderately inhibited formation intermediates glycosylation, fructosamine ?-dic...