early in this study, an α-amylase from bacillus megaterium who (bmw) was isolated from hot springs of ramsar (north of iran), and its gene was cloned in e.coli. based on its conserved sequence regions and substrate specificity, it was classified as intermediary group enzymes with the specificity of oligo-1,6-glucosidase and neopullulanase subfamilies. in the sixth conserved region (83-qvngiwmmp...

elm leaf beetle, xanthogaleruca luteola, is a serious pest (particularly at its larval stage) of elm trees in many areas. the existence of three important enzyms namely: ?-amylase, ?- glucosidase and ?-glucosidase in the midgut of the insect at its larval stage was investigated. optimum activity for ?-, ?-glucosidase and ?-amylase was recorded at phs 5.0, 6.0 and 6.0 against p-nitrophenyl-?-d-g...

the colorado potato beetle, leptinotarsa decemlineata say (col.: chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. in this study, some biochemical aspects of β-glucosidase in the colorado potato beetle were determined. the results showed that β-glucosidase activity in the midgut of adults wa...

β-Glucosidase hydrolyzes cellobiose to glucose and is an important enzyme in the consortium used for hydrolysis of cellulosic and lignocellulosic feedstocks. In the present work, β-glucosidase was covalently immobilized on non-porous magnetic particles to enable re-use of the enzyme. It was found that particles activated with cyanuric chloride and polyglutaraldehyde gave the highest bead-relate...

The fungus Penicillium purpurogenum was found to produce intracellular ß-glucosidase. Maximum activity of ß-glucosidase was observed on sucrose. Various cultural parameters of cultivation of P. purpurogenum for production of ß-glucosidase were optimized. Maximum enzyme content was observed after 96 hours of cultivation at 30°C. Addition of amino acids histidine and cysteine induced ß-glucosidas...

Early in this study, an α-Amylase from Bacillus megaterium WHO (BMW) was isolated from hot springs of Ramsar (North of Iran), and its gene was cloned in E.coli. Based on its conserved sequence regions and substrate specificity, it was classified as intermediary group enzymes with the specificity of oligo-1,6-glucosidase and neopullulanase subfamilies. In the sixth conserved re...

the leopard moth, zeuzera pyrina, is a key and worldwide pest damaging walnut as well as many other fruit trees during the larval stages. larvae were collected from infested twigs of walnut trees. midguts from larvae were removed under a stereomicroscope and their homogenates used as the enzyme source. larval midgut extracts showed an optimum activity at ph 8.0, 6.0 and 6.0 for ?-amylase, ?- an...

application of chemical pesticides has increased significantly worldwide and has raised serious concerns about environmental pollutions. one of the encouraging trends to minimize pesticide risk is production of resistant plants containing toxic proteins against insect pests. considering the importance of purification and characterization of digestive enzymes in the production of resistant plant...

An efficient and rapid affinity-based screening method for directly fishing out natural alpha-glucosidase inhibitors from Cyperus. rotundus extract by using immobilized enzyme technology combined with UHPLC-QTOF MS analysis was established. As a results, without time-consuming and laborious isolation workload and false positive interference, five natural alpha-glucosidase inhibitors were succes...