background: although cervical cancer mortality has reduced during last years, but it is still leading cause of mortality among women. many efforts have performed to develop new drugs and strategy to cure cervical cancer. mesenchymal stem cells (mscs) have many advantages that make them a suitable choice as a cell therapeutic agent in cancer treatment. there are different transfection methods fo...

objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

The resistant and susceptible tea cultivars were selected for revealing the defense response mechanism induced by blister blight. DEGs before after infection blight analyzed based on RNA sequencing digital expression spectrum. results showed a total of 974 identified, which 122 co-expressed genes in both cultivars, 364 specific only disease-resistant cultivar, 488 disease-susceptible cultivar. ...

Heritable variation in a gene’s expression arises from mutations impacting cis - and trans -acting components of its regulatory network. Here, we investigate how -regulatory are distributed within the genome gene network by identifying characterizing 69 with effects on same focal Saccharomyces cerevisiae . Relative to 1766 without this gene, found that these were enriched coding sequences trans...

The angiotensin-converting enzyme 2 (ACE2) is the receptor for three coronaviruses HCoV-NL63, SARS-CoV, and SARS-CoV-2. ACE2 involved in regulation of renin-angiotensin system blood pressure. also several signaling pathways, including integrin signaling. expression regulated transcriptionally post-transcriptionally. gene by two promoters, with usage varying among tissues. greatest small intesti...

Ethylene plays an important role in wide-ranging aspects of plant growth and development, includingfruit ripening, leaf and flower senescence. In this study, the expression patterns of two genes involved in theethylene signal transduction pathway (RhCTR1 and RhCTR2) were investigated during the flower openingstages in two Rosa hybrida cultivars, ‘Black magic’ and ‘Maroussia’, ...