a study was conducted to identify ssr markers linked to leaf rust resistance genes lr24 and lr28 and to be used for marker-assisted selection (mas) to transfer both genes to a widely cultivated wheat variety mp 3299 under rainfed condition. f2 individuals of the cross mp 3299×nil pbw 343 were used for generating genotypic data employing closely linked scar markers s73719 and s421570 to lr24 and...

Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

the green fluorescent protein (gfp) was originally isolated from the jellyfish aequorea victoria that fluoresces green when exposed to blue light. gfp protein is composed of 238 amino acids with the molecular mass of 26.9 kd. the gfp gene is frequently used in cellular and molecular biology as a reporter gene. to date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including h...

in this study, an efficient agrobacterium-mediated transformation method was developed forpomegranate (punica granatum l.), a difficult-to-transform plant. in vitro shoot segments wereinoculated with agrobacterium tumefaciens strain lba4404 harboring the binary vectorpbi121 carrying the neomycin phosphotransferase (nptii) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

With the advancement of metagenome data mining science has become focused on microarrays. Microarrays are datasets with a large number of genes that are usually irrelevant to the output class; hence, the process of gene selection or feature selection is essential. So, it follows that you can remove redundant genes and increase the speed and accuracy of classification. After applying the gene se...

The current study was conducted to consider how pedigree and the gene-dropping analysis can use to monitor genetic diversity, and to recommend a breeding strategy for improving breast weight (BRW) in a population of Japanese quail. A total of 312 birds were divided equally into two lines. One line (S1) was selected for four-week body weight (BW) based on breeding value, and the other (S2) was s...