The conversion of deuterium-labeled ceramide to cerebroside has been studied. A mixture of N-(2’+hydroxy [4’,4’,5’,5’-2H4]hexadecanoyl) D-eryfhYO-1,3-dihydroxy-2amino-4-frans-[4,5-*H2]octadecene and N-(2’-D-hydroxy hexadecanoyl) D-eryfhro-l,3-dihydroxy-2-amino-4-frans-[33Hl]octadecene was incubated with mouse brain microsomes and a UDP-galactose regenerating system. Both galactosyl ceramide and...

Tripterygium wilfordii (Hook. f.) is a woody vine of the Celastraceae family, and native to China (south of the Yangtze River), Korea, and Japan, is used in the clinic for the treatment of inflammatory and autoimmune diseases, especially rheumatoid arthritis. In this study, we developed a urine metabolomic method by gas chromatographymass spectrometry (GC-MS) to evaluate the effect of Tripteryg...

During barrel maturation, the volatile compounds extracted from oak wood can contribute to a wine’s overall aroma and flavour, enhancing its character and complexity. However, barrels have a finite pool of extractable material and the amounts of oak derived volatile compounds available for extraction diminish over time. As a consequence, barrels are typically decommissioned after five to six ye...

Methods Stem Br (Vital Nutrients lot # 2890; Middletown, CT), was tested for authenticity and purity. Female C57BL/6J mice, (Jackson Laboratory; Bar Harbor, ME) received one bolus, i.p. injection of Br (12mg/kg) in 0.5ml of physiological saline and plasma collected at baseline 3, 6, 12 and 24 hours. All procedures were approved by the Animal Care Committee at University of CT Health Center. Pos...

Rhanterium epapposum, native to the Arabian Peninsula, is traditionally used cure skin infections. The objective screen phytochemical content and antimicrobial activity of aqueous, methanol 80% extracts aerial parts R. epapposum. screening methanolic, epapposum was conducted using gas chromatographymass spectrometry. activities were assessed by well diffusion microdilution methods. Qualitative ...

In this study, olive oil residue (OR) biomass was pyrolyzed in the presence of bulk MgO (B-MgO), nano-MgO (N-MgO), ZnO (B-ZnO)), and nano-ZnO (N- ZnO) metal oxides at different temperatures (400, 600, 800 ºC). Significant results were obtained terms synthesis gas formation CO2 reduction. The efficiency distribution products as a result oxide-based pyrolysis process effects examined detail. Nano...

The essential oil from the aerial parts of Iranian and British Thymus vulgaris L. were obtained by hydro distillation and analyzed by gas chromatography (GC) and gas chromatographymass spectrometry (GC-MS). Forty two components were identified in the Iranian Th. vulgaris essential oil that represented 99.06% of the oils. The major components were thymol (60.54%), γTerpinene (9.47%), p-cymene (8...

Primary carnitine deficiency (PCD) is a rare autosomal recessive disorder, caused by deficiency in the plasma membrane carnitine transporter. In this report, we aimed to describe the diagnosis and treatment of 12 PCD patients. Blood spots were collected from the children, and analysed using liquid chromatography-mass spectrometry (LC-MS). Newborns and children who had repeated lower dried blood...

The aim of this study was to screen for microorganism that able to utilize 3-N-trimethylamino-1-propanol (homocholine) as a sole source of carbon and nitrogen. The aerobic degradation of homocholine has been found by a gram-positive Rhodococcus sp. bacterium isolated from soil. The isolate was identified as Rhodococcus sp. strain A4 based on the phenotypic features, physiologic and biochemical ...