Our search for new 3-hydroxyfusicoccins structurally related to cotylenin A from a culture of Phomopsis amygdali Niigata 2-A resulted in the isolation of novel 3-hydroxy fusicoccins, called fusicoccins R and S, and the fusicoccin S aglycon, called phomopsiol, together with known 3alpha-hydroxyfusicoccin J. The structure of phomopsiol was identified as that of O-demethyl-3-epicotylenol based on ...

Our search for new polar f"siceccins biosynthetically related to fusicoccin A from the culture filtrate of isolate Niigata 2-A of a peach Fusicoccum canker fungus resutted in the iso]atiofi of new fusicoccins named fusicocci"s P and Q, and 3-epifusicoccins H and Q, together with 3'-deacetylf"sicoccin A and 16-Odemethyl-3-epifusicoccin J. The structures of fusicoccins P and Q, and of 3-epifuslco...

The action of exogenous polyamines (putrescine, spermidine, and spermine) on ;washing' and fusicoccin-stimulated K(+) uptake and H(+) extrusion through the plasmamembrane in maize (Zea mays L., hybrid line Plenus S 516) root apical segments was studied. The results showed that polyamines inhibit the washing-stimulated K(+) influx and H(+) extrusion without interfering with K(+) uptake and H(+) ...

The plant plasma membrane H(+)-ATPase is activated by the binding of 14-3-3 protein to the C-terminal region of the enzyme, thus forming an H(+)-ATPase-14-3-3 complex that can be stabilized by the fungal toxin fusicoccin. A novel 14-3-3 binding motif, QQXYpT(948)V, at the C terminus of the H(+)-ATPase is identified and characterized, and the protein kinase activity in the plasma membrane fracti...

Systemin is an important mediator of wound-induced defense gene activation in tomato plants, and it elicits a rapid alkalinization of the growth medium of cultured Lycopersicon peruvianum cells. A possible mechanistic link between proton fluxes across the plasma membrane and the induction of defense genes was investigated by modulating plasma membrane H 1 -ATPase activity. Inhibitors of H 1 -AT...

When the protoplasts of peeled oat leaf segments (Avena sativa L.) expand after a brief plasmolysis (osmotic shock), fusicoccin-enhanced H(+) excretion is reduced and protein is released to the rehydration medium. This shock protein seems to arise from the cell surface, not from the interior of leaky cells or from broken cells, because (a) the protein differs quantitatively and qualitatively fr...

Amaranthus tricolor half-seedlings show a greater accumulation of betacyanin in response to a defined period of red light if given a pretreatment at an elevated temperature (40 C). Red light given before the shift to 40 C is ineffective. The maximum response is achieved after a 2-hour shift to 40 C and if the red light is given 1 hour after return to the germination temperature (25 C). The effe...

Halogeton glomeratus (Bieb.) C.A. Mey (Chenopodiaceae) is an annual forb that is widely distributed in mixed desert shrub, salt desert shrub and pinyon-juniper communities of northern Utah. Halogeton glomeratus seeds gave only 78% germination in distilled water. Fusicoccin (FC) had some effects in partially alleviating innate dormancy while other chemicals had no effect. Seed germination was in...